lipofection


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lip·o·fec·tion

(lip'o-fek'shŭn),
The process of injecting a lipid-complexed or contained DNA into eucaryotic cells.
[lipo- + transfection]

lipofection

using encapsulation in a phospholipid vesicle as a means of delivering RNA, DNA or other substances into a eukaryotic cell.
References in periodicals archive ?
One day after lipofection, GFP-expressing cells were sorted using a FACSAria III cell sorter (Becton, Dickinson and Co.
HSA-PEI/pEGFP had been shown to provide better pEGFP transfection efficiency than PEI/pEGFP, jetPEI/pEGFP or lipofection.
Methods: Stably RIP140-overexpressing N2a (N2a-RIP140) cells were used as a neurodevelopmental model, and were constructed by lipofection and overexpression validated by real-time polymerase chain reaction and Western blot.
Development of such a delivery system for therapeutic purposes faces many challenges, including ethical and technical issues; for example, choice of the best gene transfection method to introduce the RNA/protein of interest (cargo) into exosome-secreting cells (virus, lipofection, electroporation), how to target the cargo into the exosomes, the best technique to load exosomes with the drug of interest (electroporation or lipofection), and the cost of the process are a few of the concerns that need to be addressed.
4% was the highest transfection efficacy obtained using the lipofection method (Figure 3).
Cell analysis, the second largest life science instrumentation market, is made up of many subsegments, including electroporation and lipofection, the largest of which is flow cytometry.
Fish and chicken embryos were also transfected using lipofection, electroporation or high velocity microprojectiles (Szelei et al.
HeLa cells were transiently transfected using the lipofection technique.
We employed some physical and chemical gene transfer methods (electroporation, Lipofection and calcium phosphate) for SP2/0 transfection, but none was found to be efficient (data not presented).
2] fraction (B), EtOAC fraction (C), n-BuOH fraction (D), activated trascription of ER[alpha] or ER[alpha] in HeLa ER[beta] by lipofection.