limulus amebocyte lysate test

limulus amebocyte lysate test

(lĭm′ū-lŭs),

LAL test

A test used to detect minute quantities of bacterial endotoxins and to test for pyrogens in various materials; it is also used to detect septicemia due to gram-negative bacteria. Limulus amebocyte lysate is formed from the lysed circulating amebocytes of the horseshoe crab (Limulus polyphemus).
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Before using the GA in experiments, the GA was checked for endotoxin presence by Limulus amebocyte lysate test (E-Toxate Kit; Sigma) following the manufacturer's guidelines.
To determine whether the contaminant was endotoxin, we filled Vacutainer Tubes with saline and tested them for endotoxin by the Limulus Amebocyte Lysate test (Bio-Whittaker, Inc.
The presence of endotoxin in the condensed GSE was checked by Limulus amebocyte lysate test (E-Toxate Kit; Sigma).