Structures of the cerebral nuclei and the limbic lobe were chosen because they showed higher expression in a preliminary study performed with eight genes of DSCR (13).
To rank the expression in several brain substructures of cerebral nuclei and limbic lobe for each one of genes a Principal Components Analysis (PCA) was performed reducing the R space from 19 coliner variables to 6 principal components ([R.
Differential expression of DSCR in human brain A quantitative analysis of global transcription of DSCR genes along the structures of the brain nuclei and the limbic lobe revealed that the highest values of z-score were registered in the area that includes the putamen for DSCR3, RCAN1 as well as in left and right caudate nuclei for DSCR6, and the left and right globus pallidus for DSCR3, SH3GBR, DYRK1A, CLIC6 and PRMT2.
A Cluster Analysis (CA), using the median distance values, revealed that the expression of some genes was produced in association with a series of sub structures both from cerebral nuclei and limbic lobe (Fig.
For the Limbic Lobe, six components were discriminated that explained 76.
On the other hand, for the limbic lobe the analysis also showed two associations in which the first one included DSCR2, 5 and 6 whereas the second one grouped DSCAM and DSCR4 (Fig.
One example of it was the overexpression of RCAN1, RUNX1 in contrast with the underexpression of DYRK1A that occur in caudate nuclei head left and right, putamen, internal and external globus pallidus of normal brain Such result, lead us to propose that in normal brain, expression of these genes in specific structures of cerebral nuclei, and limbic lobe regulate not only cognitive function but emotional ones via a complex network of gene expression responsible by the learning and memory functions among others associated process (23,24).
Based on those findings, the authors hypothesized that these changes or abnormalities may have been linked to excessive electrical input from the amygdala region of the brain to the hippocampus, which is part of the limbic lobe and plays an important role in emotional stress and learning.
Benes said the work means that the animal model can be used to learn more about how limbic lobe circuitry functions under both normal and abnormal conditions.