light microscope


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microscope

 [mi´kro-skōp]
an instrument used to obtain an enlarged image of small objects and reveal details of structure not otherwise distinguishable.
The light path of a darkfield microscope. From Hart and Shears, 1997.
acoustic microscope one using very high frequency ultrasound waves, which are focused on the object; the reflected beam is converted to an image by electronic processing.
binocular microscope one with two eyepieces, permitting use of both eyes simultaneously.
compound microscope one consisting of two lens systems whereby the image formed by the system near the object is magnified by the one nearer the eye.
darkfield microscope one so constructed that illumination is from the side of the field so that details appear light against a dark background.
electron microscope one in which an electron beam, instead of light, forms an image for viewing, allowing much greater magnification and resolution. The image may be viewed on a fluorescent screen or may be photographed. Types include scanning and transmission electron microscopes.
fluorescence microscope one used for the examination of specimens stained with fluorochromes or fluorochrome complexes, e.g., a fluorescein-labeled antibody, which fluoresces in ultraviolet light.
light microscope one in which the specimen is viewed under ordinary illumination.
operating microscope one designed for use in performance of delicate surgical procedures, e.g., on the middle ear or small vessels of the heart.
phase microscope (phase-contrast microscope) a microscope that alters the phase relationships of the light passing through and that passing around the object, the contrast permitting visualization of the object without the necessity for staining or other special preparation.
scanning electron microscope (SEM) an electron microscope that produces a high magnification image of the surface of a metal-coated specimen by scanning an electron beam and building an image from the electrons reflected at each point.
simple microscope one that consists of a single lens.
slit lamp microscope a corneal microscope with a special attachment that permits examination of the endothelium on the posterior surface of the cornea.
stereoscopic microscope a binocular microscope modified to give a three-dimensional view of the specimen.
transmission electron microscope (TEM) an electron microscope that produces highly magnified images of ultrathin tissue sections or other specimens. An electron beam passes through the metal-impregnated specimen and is focused by magnetic lenses into an image.
x-ray microscope one in which x-rays are used instead of light, the image usually being reproduced on film.

light mi·cro·scope

a class of microscope that forms a magnified image using visible light.

light microscope

Etymology: AS, leoht + Gk, mikros, small, skopein, to view
a microscope that uses visible light to view objects too small for the naked eye to see.

light mi·cro·scope

(līt mīkrŏ-skōp)
Class of microscope that forms a magnified image using visible light.

microscope

an instrument used to obtain an enlarged image of small objects and reveal details of structure not otherwise distinguishable.

acoustic microscope
one using very high frequency ultrasound waves, which are focused on the object; the reflected beam is converted to an image by electronic processing.
binocular microscope
one with two eyepieces, permitting use of both eyes simultaneously.
bright-field microscope
the standard bench microscope used in histology and requiring stained tissue sections.
compound microscope
the standard laboratory microscope used in veterinary science; consists of a two lens system whereby the image formed by the system near the object (objective) is magnified by the one nearer the eye (eyepiece).
darkfield microscope
used for examining unstained, often living cells, in which light is only directed into the objective lens if it is deflected by an object in its path. The object is thus viewed as a white structure in an otherwise black (darkfield) background.
electron microscope
one using an electron beam of very short wavelength as the source of illumination. It has a resolving power of 2 nm (which is 100 times greater than with the light microscope). Includes the transmission electron microscope and the scanning electron microscope (below). See also immunoelectron microscopy.
fluorescence microscope
one used for the examination of specimens stained with fluorochromes or fluorochrome complexes, e.g. a fluorescein-labeled antibody, which fluoresces in ultraviolet light. See also fluorescence microscopy.
interference microscope
a microscope similar to the phase contrast microscope but delivers a three-dimensional image. Called also Nomarski interference phase microscope.
light microscope
used for examining unstained or stained particles or the cellular structure of tissues that have been cut into sections and stained. It has a resolving power of 0.2 μm. Modern light microscopes have an eyepiece and objective lenses which provide magnification, and a condenser beneath the stage which gathers and focuses light on the object being examined.
operating microscope
one designed for use in performance of delicate surgical procedures, e.g. on the middle ear, eye or small vessels of the heart.
phase microscope, phase-contrast microscope
a form of light microscope useful for examining living, unstained structures, including animal cells and bacteria, e.g. leptospira. The phase of the light wave passing through different structures in the cell, e.g. nucleus vs. thin part of the cytoplasm, is changed by different amounts and thereby provides contrast.
polarizing microscope
based on the phenomenon of birefringence; useful in the study of bone and muscle.
scanning electron microscope (SEM)
an electron microscope that produces a high-magnification image of the surface of a metal-coated specimen (shadow casting) by scanning an electron beam and building up an image from the electrons reflected at each point. Particularly useful for determining the three-dimensional structure of objects.
simple microscope
one that consists of a single lens.
specular microscope
one used in the examination of the corneal endothelium.
stereoscopic microscope
a binocular microscope modified to give a three-dimensional view of the specimen.
surgical microscope
see operating microscope (above).
transmission electron microscope, TEM
one that resembles an inverted light microscope in that the beam of electrons generated from a heated filament at the top of the instrument passes down through a column where it is focused by magnetic coils (lenses) and is differentially scattered when it passes through the specimen. The image is recorded either on a photographic plate or on a phosphorescent screen.
ultraviolet microscope
uses an ultraviolet light source; useful in histochemical studies; only photographic images are available.
References in periodicals archive ?
Schmidt began to systematically observe animal cells and tissues with the polarized light microscope.
Emphasis is placed on proper illumination, calibration and preliminary observations made with stereomicroscopes and polarized light microscopes (PLM), which are the instruments typically used first in any fibrous evidence examinations.
For high-magnification work, a more shallow etch depth is desired due to the more limited depth of field of the light microscope, while for low-magnification examination a deeper contrast etch is best.
The new microscope is about 500 times more powerful than ordinary light microscopes like the ones you use at school.
Which of the regional markets represents most of the growth opportunities for the light microscope, electron microscope and scanning probe microscope markets?
Tender notice number : IRCER 17 18 P and S 20 B Inverted Light Microscope
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A high-definition, three-dimensional light microscope captured this unusual view of sugar crystallizing out of a solution.
The multiple-magnification light microscope can employ various contrast methods, including fluorescent and polarized light.
Investigators can leverage the strengths of each platform, for example, in using the resolving power of electron microscopy to image structures localized by fluorescent tags in a light microscope.
The IlluminatIR[TM] conveniently mounts between the nose-piece and viewing head of a light microscope and provides immediate and accurate molecular information, helping to significantly speed up completion of analytical testing.
The exudate may then be collected and prepared for examination in one of three ways: 1) Draw lesion aspirates into the barrel of the needle with a tuberculin syringe and cap the needle (4); 2) touch a light microscope slide to the vesicle fluid; or 3) touch a 400-mesh, plastic-coated specimen grid directly to the base of the lesion (5).