5 [micro]g/ml leupeptin
, 1 mM phenylmethanesulfonyl fluoride (PMSF, Beyotime, Jiangsu, China).
1% sodium dodecyl sulfate, 150 mM NaCl, 1 mM phenylmethylsulfonyl fluoride, 1 gg/mL leupeptin
In this separate study to characterize secreted and circulating forms of BNP, coronary sinus and matching femoral artery samples were collected into chilled 9-mL glass tubes containing 140 [micro]g EDTA, 380 [micro]g leupeptin
, and 5.
The objective of present work was to design novel antimalarial derivatives by generating key interaction site and receptor-based pharmacophore for already bound leupeptin
order to generate its analogues leading to better inhibitors for deadly disease malaria.
, a calpain inhibitor, protects inner ear hair cells from aminoglycoside ototoxicity.
A, 1 mM phenylmethylsulforiyl fluoride, 1 [micro] g/mL aprotinin, 1 [micro] g/mL leupeptin
, 1 [micro] g/mL pepstatin, 1 mM [Na.
The cells were then treated with 1 ml of a 1% solution of Triton X-100 (containing 5 [micro]g/ml each of antipain, pepstatin, and leupeptin
as well as 1 mM phenylmethylsulfonyl fluoride) and kept at on ice for 20 min.
, a protease inhibitor, decreases protein degradation in normal and diseased muscles.
0004% w/v each of antipain, leupeptin
, pepstatin A, chymostatin).
Samples to be analyzed for protease-sensitive PrP (PrPsen) contained the following protease inhibitors: 10 [micro]mol/L leupeptin
, 1 [micro]mol/L pepstatin A, and 1 [micro]g/mL aprotinin.
Soya bean trypsin inhibitor (STI), fatty acid free bovine serum albumin (BSA), pyruvate kinase (PK), leupeptin
, ATP, ADP, PCr, ethylene glycol-bis(2-aminoethyl)-N,N,N'N'-tetraacetic acid (EGTA), ethylenediaminetetraacetic acid (EDTA), and 4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid (HEPES) were from Roche, N,N-bis(2-hydroxyethyl)2-aminoethanesulphonic acid (BES), Cr, and other reagent grade chemicals used were from Fluka or Sigma.
Inhibitory effect of a protease inhibitor, leupeptin
, on the development of influenza pneumonia, mediated by concomitant bacteria.