LAD is a rare immune deficiency disorder and should be suspected in an infant with typical presentation and confirmed by flow cytometric analysis for the expression of deficient integrin molecules on leukocytes
In addition to the microfluidic "capture chamber," the new chip incorporates separate ports for lysing reagents and quenching buffers that preserve the leukocytes
for counting by the microfabricated electrodes.
For analysis of noncultured leukocytes
, 100 [micro]L of freshly drawn whole blood was labeled with fluorescence-conjugated antibodies for 30 min at RT, red blood cells were lysed, and the remaining leukocytes
were washed and resuspended in BD FACSflow Sheath Fluid (BD Biosciences) with 1% paraformaldehyde.
labelling of leukocytes
is a laborious process that takes about 3 hours
Serum separator tubes have been shown to contain fewer leukocytes
in the supernatant after centrifugation than corresponding PSTs (5).
Although several previous studies have investigated biological variations of numerical parameters of leukocytes
(14-17) and demonstrated hour-to-hour and day-to-day intraindividual fluctuation of WBC, (17) this study is the first to investigate the biological variations of morphologic parameters of leukocytes
because of their increasing clinical significance.
Effect of tranexamic acid on the metabolism of glutathione in leukocytes
or soluble factors released by leukocytes
during storage have been extensively studied in recent years (23).
Table 2 includes the data needed to test the research hypothesis based on the effect of an exhaustive practice as well as methylphenidate usage over the number of rats' leukocytes
and compare the average of leukocytes
in witness, exercise, 25%[LD.
The number of rolling, adherent and emigrated leukocytes
was determined off-line during playback of videotaped images.
In order to demonstrate the kinds of leukocytes
separated by Ficoll density gradients, non-induced samples were used to produce microscopy slides in a cytological centrifuge Fanem[R] model 248.
Studies have been conducted on lower vertebrates in order to understand biological roles of leukocytes
in defense mechanisms, and to establish phylogenetic studies and new experimental models.