Several human diseases are due to Leptospire
So, in the present study, an IgM-Dot ELISA in a strip form for the detection of leptospire
specific IgM antibodies has been standardized and evaluated.
MAT uses whole leptospire
as antigen and detects both lg M and lg G antibodies.
All patients had blood samples collected at the hospital for leptospire
culture and serologic testing; convalescent-phase serum samples were obtained during follow-up for 509 (73%) patients.
were cultured in Ellinghausen-McCullough-Johnson-Harris (EMJH; Difco Laboratories, Detroit, MI, USA) medium with 10% normal rabbit serum (Gibco, Carlsbad, CA, USA) at 30[degrees]C for 7 days.
Detection of pathogenic leptospires
by real-time quantitative PCR.
The MAT was performed with a panel of live leptospires
All the pathogenic leptospires
were formerly classified as members of the species Leptospira interrogans; the genus has recently been reorganised and pathogenic leptospires
are now identified in several species of Leptospira.
presence of carrier animals, environment suitable for the survival of leptospires
and behavioural and occupational factors of people that predispose them to leptospirosis are common, the magnitude and nature of these factors vary from community to community.
Laboratory tests confirmed the presence of pathogenic leptospires
in the lake; however, the scientists were not able to identify the specific animal source.
A severely under-diagnosed disease, leptospirosis is transmitted between animals in spiral-shaped bacteria called leptospires
live in the kidneys of many mammalian hosts, including rodents, insectivores, and livestock.