The following inclusion criteria were used: residing continuously for more than one year in the study area, no history of symptomatic leishmaniasis, and no contraindications to blood extraction or to administration of a leishmanin skin test (LST).
The leishmanin test: Montenegro's antigen contains a lysate of promastigotes of L.
Confirmation of diagnosis for VL in the study patients was carried out by (1) Demonstration of amastigote stage of parasite in aspirate material from lymph node, bone marrow, or spleen using direct light microscopy or (2) Positive serology using Direct Agglutination Test (DAT) at a significant titer of 1:6400 or more, combined with a negative Leishmanin
Skin Test (LST).
Furthermore, >50% of samples with positive leishmanin
test belonged to the individuals younger than 30 are of the most active group of the population due to their occupation, education, and activity.
Use of the leishmanin
skin test and Western blot analysis for epidemiological studies in visceral leishmaniasis areas: experience in a highly endemic focus in AlpesMaritimes (France).
The leishmanin skin test (LST) (Montenegro test), an intradermal injection of a suspension of killed promastigotes, measures delayed hypersensitivity reactions and appears to be the only screening test capable of detecting asymptomatic leishmania infections.
Seroepidemiological and leishmanin skin test surveys of visceral leishmaniasis in south and southwest Ethiopia.
Longitudinal study of dogs living in ana rea of Spain highly endemic for leishmaniasis by serologic analisis and the leishmanin
The leishmanin skin test, also known as the Montenegro skin test, which is negative during active infection, becomes positive a few weeks to 2 years after cure and remains positive for life.
The leishmanin skin test is positive after 2 to 3 months of infection (skin tests are not approved for use in the United States).
for the skin test was prepared by the Pasteur Institute; 0.
6%) of the 170 patients showed a positive result on a leishmanin