Legumin, vicilin, trypsin inhibitor, and lectin were detected by indirect ELISA in the various chromatographic fractions.
1] (MW 11 000), whereas convicilin (MW 71 000), vicilin (main constitutive polypeptides MW ~47 000, 33 000) and legumin ([Alpha] polypeptides MW ~45 000, [Beta] polypeptides MW ~25 000) were eluted in the Peaks 4 (a and b) and 5 (a and b).
ELISA results were consistent with SDS-PAGE patterns; legumin eluted mainly in Peak 5 and vicilin and convicilin eluted in Peak 4 (a and b) (Fig.
Regulation of legumin levels in developing pea seeds under conditions of sulfur deficiency.
Developmental and environmental regulation of pea legumin genes in transgenic tobacco.