1 * S, small; M, medium; PRGV, Pergamino virus; ANDV, Andes virus; MACV, Maciel virus; BMJV, Bermejo virus; LECV, Lechiguanas
virus; ORNV, Oran virus; CASV, Castelo dos Sonhos virus; PARV, Paranoa virus; ; NA, sufficient sequence not available for comparison; ARAV, Araraquara virus; JABV, Jabora virus; ARCV, Araucaria virus; CATV, Catacamas virus; PDOV, El Moro Canyon virus; CHOV, Choclo virus; NEMV, Neembucu virus; ALPV, Alta Paraguay virus; ITAPV, Itaporanga virus; JUQV, Juquitiba virus; LNV, Laguna Negra virus; RIOMV, Rio Mamore virus; SNV, Sin Nombre virus; ELMCV, El Moro Canyon virus; MAPv, Maporal virus; CADV, Cano Delgadito virus.
Related but different hantaviruses were found in other areas, with names that reflect the site where they were first identified: New York-1, Black Creek Canal (in Florida), Bayou (Louisiana and Texas), Andes (Argentina), Juquitiba (Brazil), Oran (Argentina), Lechiguanas
The JUQ-like clade is sister to a clade formed by Hu39694, Neembucu, Bermejo, Andes Central Plata, Lechiguanas
, and Oran; however, this relationship is weakly supported.
Six pathogenic Andes virus (ANDV) lineages that cause HPS have so far been found to circulate in Argentina in 3 HPS-endemic areas: Oran and Bermejo (BMJ) in the north; Lechiguanas
(LEC), Hu39694, and AND Cent (Central) Plata in the central provinces; and ANDV in the South (4-7).
A 303-nt fragment of the G2 gene was amplified and sequenced (bases corresponding to position 2807 to 3109 of Lechiguanas
Several distinct hantaviruses have been associated with HPS, including Andes virus (Argentina, Bolivia, Chile, and Uruguay), Bermejo virus (Bolivia), Juquitiba virus (Brazil), Laguna Negra virus (Bolivia, Paraguay), Lechiguanas
virus (Argentina), and Oran virus (Argentina) (22,23,25,28,30,31).
In Central and South Argentina, where the genotypes Lechiguanas
, Hu39694, and Andes are the most important, seroprevalence was also low, varying from 0.
1% to 15%) (39) and some American hantaviruses (Laguna Negra in Bolivia and Paraguay, Choclo in Panama, Oran and Lechiguanas
in Argentina) for which seroprevalence is high and case-fatality ratios are <30% (9,13,15).
Briefly, the IgG ELISAs were performed by coating polyvinyl chloride microtiter plates (Dynex Technologies, Chantilly, VA) overnight at 4[degrees]C with a Lechiguanas
virus (LECV) antigen (inactivated, 3 M rad gamma-ray irradiation detergent-extracted lysate of Vero-E6 infected cells, with a 100% infection index controlled by indirect immunofluorescence).
Two species of Oligoryzomys are associated with the genotypes causing hantavirus pulmonary syndrome, Oligoryzomys flavescens for Lechiguanas