Turning natures' machinery on its head, the researchers used known techniques to glue the "shoulders" of kinesin
motors to a glass substrate.
No one who sees a kinesin
in action can forget what they've seen.
CENP-E is a mitotic kinesin
directly involved in coupling the mechanics of mitosis with the mitotic checkpoint signaling machinery, regulating cell-cycle transition from metaphase to anaphase.
Since it had been already observed that in stable microtubules there is no possibility for tubulin bound nucleotide cycling, we propose that tubulin tail energase action releases the energy accumulated in metastable conformational states of kinesin
, dynein, or phosphorylated MAPs.
Instead of having kinesin
move around, the scientists anchored the molecules so that their heads would propel free microtubules through the channels, explains biophysicist Cees Dekker.
Under the agreement terms, Eli Lilly will exclusively develop and market mitotic kinesin
Eg5 worldwide except in Japan.
Since the microtubules in the case of the Munich researchers are in constant motion alongside each other due to the activity of the kinesin
molecules, the faults also migrate.
Specific topics include cell-receptor interactions, regulatory mechanisms of kinesin
and myosin motor proteins (inspiration of improved control of nanomachines), neuromechanics (the role of tension in neuronal growth and memory); roughness-induced superhydrophobicity, hierarchical carbon-based materials, nanotwinned hierarchical metals; in situ TEM electromechnical testing of nanowires and nanotubes, nano-cells for live-cell imaging of gene expression; and mechanics of curvilinear electronics, modeling and simulation of hierarchical protein materials, and multiscale modeling for the vascular transport of nanoparticles.
All rights to ispinesib and SB-743921, each novel inhibitors of kinesin
spindle protein (KSP), will revert to Cytokinetics, on agreed terms.
The 17 papers here reflect these and other advances, including new technologies of detection and detection of resistance, physical mapping of genomes, genome-based identification and molecular analysis, microarray analysis in genetic research, manipulation of large bacterial DNA segments, amplified fragment length polymorphism analysis, phages and prophages (and their practical aspects), applications of cell imaging, kinesin
accumulation, transport assays in intact cells, methods in cell-to-cell signaling, development of strains as cancer therapy agents, and further resources for molecular protocols in research.
The concern of copurifying a contaminating activity from the expression host is also eliminated since bacteria, in contrast to the bacculovirus expression system, do not have kinesin
Technological advances in biology and physics are revving up research on kinesin
and myosin, the two major kinds of motor proteins, Building on research during the last three decades, several groups are now "setting the stage to solve the mechanism by which these biological motors move," says Steven M.