isoschizomer


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i·so·schiz·o·mer

(ī'sō-skiz'ō-mĕr),
A restriction endonuclease from different organisms that recognizes and hydrolyzes at the same DNA sequence.
[jiso- + G. schizō to split, + -mer]

isoschizomer

a RESTRICTION ENZYME that recognizes the same recognition sequence for cleavage of the DNA as other restriction enzymes.
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References in periodicals archive ?
In both methods, the DNA was digested by HpaII (a methylation-sensitive enzyme) and MspI (a methylation-insensitive isoschizomer enzyme) and analyzed by real-time PCR amplification.
To interpret the H3 motif, primers CV140F and CV120R were designed to introduce a FokI site (an isoschizomer of BstF5I) in the amplified product by substituting the restriction recognition sequence GGATG for T (nucleotide -147) and G (nucleotide -110), respectively.
The amplified samples were checked for purity and quantity on a standard agarose gel (10 g/L) and digested with Cfol, an isoschizomer of Hhal.
tuoliensis was digested with isoschizomers Msp I or Hpa II (mixture of EcoR I), the ability to digest the sequence CpCpGpG as influenced by their methylation state.
Thereafter, the genomic DNA was digested with the isoschizomers HpaII and MspI.
Among the topics are detecting changes in global genome methylation using the cytosine-extension assay, isoschizomers and amplified fragment length polymorphism for detecting specific cytosine methylation changes, northern blotting techniques for small RNAs, cloning new small RNA sequences, cDNA libraries for virus-induced gene silencing, and detecting and quantifying DNA strand breaks using the random oligonucleotide primed synthesis (ROPS) assay.