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isoelectric focusing |
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focusing /fo·cus·ing/ (-ing) the act of converging at a point. isoelectric focusing electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point. isoelectric focusing, the ordering and concentration of substances according to their isoelectric points. isoelectric focusing, n the ordering and concentration of substances according to their isoelectric points. focusing the act of converging at a point. isoelectric focusing electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site at which the pH is equal to its isoelectric point. isoelectric showing no variation in electric potential. isoelectric focusing an electrophoretic method for separating proteins in gels which depends on the fact that the net charge on the protein varies with the pH of the surrounding medium. At the isoelectric point the protein has no net charge and will not therefore migrate further in an electric field. The polyacrylamide gel is cast in a narrow tube that provides a pH gradient from top to bottom. isoelectric period the moment in muscular contraction when no deflection of the galvanometer is produced. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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coli CO subjected to isoelectric focusing analysis showed 3 [beta]-lactamase activities with pI values of 5. The new system, which fully automates all processes from isoelectric focusing to polyacrylamide gel electrophoresis to detection, can perform two-dimensional electrophoresis of proteins in about one hour. Handbook of Isoelectric Focusing and Proteomics David Garfin, Satinder Ahuja Burlington, MA: Elsevier, 2005. |
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