isoamyl alcohol


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Related to isoamyl alcohol: isopropanol, Isoamyl acetate

isoamyl alcohol

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Phenol: Chloroform: Isoamyl alcohol (25:24:1v/v/v),
Transfer the upper aqueous phase carefully to a new tube and mix with an equal volume of chloroform: isoamyl alcohol (24:1) by inversion and centrifuge at 10, 000 g for 10 min at room temperature to separate the phases.
The decrease in the proportion of hexane (57%), the addition of a more polar solvent, dichloromethane (42%), and isoamyl alcohol (1%) to break up emulsions was shown to be preferable for recovery of tricyclic ADPs and fluoxetine, as we previously described in recent reports [5, 6].
2]O and boiled for 10 min, then centrifuged at 1,000 x g for 2 min and the supernatant containing the DNA extracted twice with phenol: chloroform: isoamyl alcohol (25:24:1) and precipitated with ethanol.