Agrivida will bolster its existing technology portfolio, including its proprietary intein trait platform, with technology licensed from Syngenta that will be used to develop new traits for multiple crops, including corn, sorghum, switchgrass and miscanthus.
The combination of Agrivida's proprietary intein platform with Syngenta's technology will allow us to provide an integrated solution for feedstock and enzyme delivery to a wide range of industrial customers.
coli as a fusion protein with an intein tag that contains a ch itin-binding domain.
After restriction digestion, the amplified fragments were inserted to yield a C-terminal in-frame translational fusion with an intein tag that features a chitin-binding domain.
coli as an in-frame translational fusion with a self-cleavable intein with a chitin-binding domain.
A small portion of RecA, dubbed the intein, quickly cuts itself out of the protein and melds the two remaining fragments together to form the final, active molecule.
Despite this rarity, scientists have learned a lot over the past decade about the mechanics of intein action.
Scientists understand the role of RecA and realize that it has an intein, but they have no idea whether cells have developed ways to take advantage of the presence of this intein or any other.
Since the researchers reported this proof that protein splicing happens, investigators have worked to describe the steps by which an intein cleaves the chemical bonds that hold it within the precursor protein and then seamlessly joins the protein fragments from which it has escaped.
Scientists needing to collect large quantities of a particular protein can take the gene for that protein and fuse it to the DNA sequence encoding a modified intein.
These hybrids are easy to separate from other bacterial molecules because the company engineered the intein to stick to a protein called chitin.