39) Segmental labeling by either intein splicing reaction or chemical ligation (40,41) was reported.
After removal of denaturant, the intein with a nick was renatured (Fig.
Fused genes of N- and C-terminal fragments of [beta] subunit and intein in pET vectors were expressed in E.
Pharmavite LLC offers Natare Made Eye Defense, which is formulated with intein
and zeaxanthin--another carotenoid--that help to maintain a healthy macula.
The expressed protein was intein
tagged and purified by binding the tagged protein to a chitin column and then performing an on-column cleavage of the intein
coli as a fusion protein with an intein tag that contains a ch itin-binding domain.
After restriction digestion, the amplified fragments were inserted to yield a C-terminal in-frame translational fusion with an intein tag that features a chitin-binding domain.
coli as an in-frame translational fusion with a self-cleavable intein with a chitin-binding domain.
A small portion of RecA, dubbed the intein, quickly cuts itself out of the protein and melds the two remaining fragments together to form the final, active molecule.
Despite this rarity, scientists have learned a lot over the past decade about the mechanics of intein action.
Scientists understand the role of RecA and realize that it has an intein, but they have no idea whether cells have developed ways to take advantage of the presence of this intein or any other.
technology has been pioneered and developed by scientists at NEB for various biotechnology applications including the expression and downstream purification of recombinant proteins.