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insertional

   Also found in: Dictionary/thesaurus, Legal, Encyclopedia, Wikipedia 0.03 sec.
insertional
pertaining to
1. surgical or reproductive manipulation
2. molecular biological manipulation.

insertional activity
the electrical tracing produced in electromyography as a result of the insertion of the needle electrode.
insertional inactivation
in recombinant DNA technology, an early method for selecting ampicillin (amp+) and tetracycline (tet+) resistant Escherichia coli transformed with plasmids such as pBR 322; foreign DNA was cloned into the tet gene, thereby disrupting the gene and rendering the cell tet, which enabled selection when cells were plated onto amp and tet plates.


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Needle electromyography (EMG) of bilateral tibialis anterior and peroneus longus muscles revealed motor unit potentials of normal amplitude, duration, and phasicity; increased insertional activity, 2+ fibrillations, 2+ positive sharp waves and reduced recruitment.
The papers focus on a range of chromosomal mutagenesis techniques for both prokaryotic and eukaryotic organisms, and present a variety of state-of-the-art methods in step-by-step laboratory format, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and novel tools based on integrases, eukaryotic transposons, triplex forming oligonucleotides, group II introns, and engineered site-directed nucleases.
 
 
 
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