insertion sequence


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Related to insertion sequence: Inverted repeat, transposase

in·ser·tion se·quence

discrete DNA sequences of nucleotides that are repeated at various sites on bacterial chromosomes, certain plasmids, and bacteriophages and that can move from one site to another on the chromosome, to another plasmid in the same bacterium, or to a bacteriophage.

insertion sequence (IS)

a type of TRANSPOSABLE GENETIC ELEMENT that codes for its own transposition but which apparently carries no other genetic information. Generally it is several hundred base pairs in length. It is capable of causing MUTATION when it inserts into a GENE. Insertion sequences may exist as independent entities or may form the terminal parts of certain TRANSPOSONS.
References in periodicals archive ?
tuberculosis isolates, the use of insertion sequence IS6110 is the most widely used method.
After removing the insertion sequences at the hemagglutinin (HA) cleavage sites from HPAI viruses, we concatenated all available whole-genome sequences and analyzed them by using the median-joining method implemented by NETWORK version 5.
14-2 2013 -- pEc 20COE13 ([section]) 2014 -- pBA77-MCR-1 2015 1294 pP111 -- * UAE, United Arab Emirates; --,no known insertion sequences were identified.
rmtE1 is bound by an IS CR20-like element and an IS 1294-like insertion sequence.
meningitidis adhesin A variant had an insertion sequence that disrupted this gene, as described for ET-15 meningococci (10).
Moreover, the genetic environment of fosA3 was assessed by PCR mapping and sequencing (2,6), showing a composite transposon containing an insertion sequence 26 323 bp upstream fosA3; the orfl, orf2, and orf3 genes (homologous to regulatory ones in K.
The LV143 strain was positive for the insertion sequence ISEcp1, associated with [bla.
qPCR targeting the repeated insertion sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8).
Analysis by mycobacterial interspersed repetitive unit-variable number tandem repeats (15) identified 2 strain variants in our sample set (Table 2); IS6110 DNA fingerprint analysis (12) of isolate MK172 showed it to contain 21 copies of the IS6110 insertion sequence element (Figure 1).
sonnei (Ss046) it is inactivated by a frameshift mutation and an insertion sequence, respectively (12).