impression cytology

cytology, impression 

A simple, non-invasive means of studying cells on the conjunctiva. It is carried out by pressing a small piece of special filter paper against the anaesthetized bulbar conjunctiva for a few seconds, after which it is removed. The operation is usually repeated two or three times over the same area. The filter paper is then fixed to a glass slide, stained and examined under a microscope. Mucin, goblet cells and many epithelial cells which stain in different colours, depending on the dyes used, can be assessed and facilitate the diagnosis of many external eye diseases, such as keratoconjunctivitis sicca and xerophthalmia. See Gram stain.
References in periodicals archive ?
The most widely used diagnostic tests for tear film examination are tear break-up time (TBUT) test, corneal and conjunctival staining with fluorescein, rose bengal and lissamine green dye, Schirmer test, tear film osmolarity test and impression cytology.
Impression cytology refers to the application of cellulose acetate filter paper to the ocular surface to remove the superficial layers of the ocular surface epithelium.
Ocular Surface Disease Index (OSDI) questionnaire, break-up time (BUT) test, corneal fluorescein staining, corneal and conjunctival rose Bengal staining, Schirmer test, and conjunctiva impression cytology were performed sequentially on days 0 and 91.
Conjunctiva impression cytology is one of the most practical, fast, safe and economical method to examine the changes of conjunctiva cells.
9) Superficial excision remains the important initial step in management, as it is impossible to exclude invasive disease on clinical grounds or with impression cytology.
The technique of impression cytology was first described by Egbert et al in 1977.
Dry eye was confirmed by ocular surface dye staining pattern with fluorescein, tear film break up time (value 15s), Schirmer's test (positive if 10mm or less in 5min) and conjunctival impression cytology.