Ethidium Bromide and Propidium Iodide are classic cell impermeant
DNA stains that are structurally similar to phenanthridium intercalators.
15] presented a theoretical model that determined membrane pore size as a function of calcein (a cell impermeant
dye) uptake where calcein uptake is directly related to pore size (i.
Since propidium iodide, a dye highly impermeant
to intact plasma membranes, is unable to stain living cells, K562 cells exerting propidium fluorescence are dead or have compromised membranes.
Lithium uptake was measured in artificial seawater (ASW) solutions with Na replaced with the impermeant
cation choline and lithium (100% ASW assay solution: 9.
The gating currents were isolated by replacing permeant ions with impermeant
ones, thus reducing ionic currents, subtracting away symmetrical currents and, in most cases, blocking the ionic sodium current with tetrodotoxin [1, 2].
The medium was then replaced by fresh culture medium without serum nor antibiotics and the cells - with exception of the SH-SY5Y which were directly submitted to the next step - were further incubated for 30 min to allow full hydrolysis of Fura-2/acetoxymethyl by cellular esterases, resulting in cytosolic capture of the membrane impermeant
The inhibition of hexose transport by permeant and impermeant
sulfhydryl agents in rat adipocytes.
Although an in vivo calibration following application of quinidine could not be performed, an estimate of the change in intracellular sodium as a result of quinidine application, based on the calibration of the instrumentation with the cell impermeant
form of SBFI, reveals an increase of approximately 102 [+ or -] 6 mM (SE) intracellular sodium over baseline levels.
Red cell membranes enclose high concentrations of impermeant
anions, principally Hb (5 mmol/L), 2,3-diphosphoglycerate (DPG; 5 mmol/L), and glutathione (2-3 mmol/L), and any osmotic imbalance creates an intolerable osmotic gradient, allowing the instant entry of water.