In the vehicle-treated group, cells co-labeled with BrdU and NeuN immunoreaction
, as newly generated neurons, were mainly detected in the SGZ of the DG [Figure 3]a, [Figure 3]b, [Figure 3]c, and the mean number of BrdU +/NeuN + cells was 7.
1990) identified viral particles in three cats with olfactory neuroblastoma that, through IHC, demonstrated an immunoreaction
in the cytoplasm of neoplastic cells for FeLV, similar to what was observed in our case.
The myocardial sections stained with caspase-3 immunostain showed positive brown cytoplasmic caspase-3 immunoreactivity (Figure 2(b)), while sections stained with KI67 immunostain showed negative brown nuclear immunoreaction
In other cells/areas, immunoreaction
for AGE was invariably positive, such as fat cells, vascular endothelial cells, dermal collagen fibers (on superficial dermis 2+/3+, on deeper dermis 3+), and perivascular collagen.
Prion protein immunostaining showed intense immunoreaction
of the core of the florid plaques and patchy and granular deposits that were arranged in rounded clusters (Figure 3, panel C).
Positive reaction involves intensive nuclear and cytoplasmic immunoreaction
was amplified with the avidin-biotin complex and visualized with 3,3'-diaminobenzidine.
As previous studies have demonstrated, there was a strong immunoreaction
of antihuman PR antibody against PR in crustaceans based on a conservative region of the PR protein chain (Coccia et al.
The percentage of apoptosis obtained through TUNEL and immunoreaction
assay does not show differences in cells infected with the strains V70/06 and V33/98 of the Sp genotype and strain V112/06 of genotype VR-299, evidencing that the genotype of the strain is not related to this form of cell death.
Electrochemiluminescence reagent was then used to determine the immunoreaction
and was measured by densitometry on an X-ray film, using the GIS software (Bio-Tanon, China).
The length of fixation also affects the results of immunostaining as underfixation often produces a reduced immunostaining in the central region of the tissue block with stronger immunoreaction
in the marginal area of the section while overfixation generates the opposite effect (13).
Furthermore, AC binds to plasma proteins such as apolipoprotein E, transthyretin, complement proteins, albumin, and A[beta]-specific auto-antibodies, thereby masking epitopes that may interfere with the ELISA immunoreaction