For example, to determine whether an observed interaction is really genuine or just results from the experimental conditions, one can perform a reverse immunoprecipitation
(Maiya et al.
As shown in Figure 4, SOHLH1 was detected in NGN3 complexes immune precipitated from 7week-old mice testis extracts, and NGN3 was also confirmed in the reciprocal SOHLH1 immunoprecipitation
To examine the kinsenoside-mediated genomic regulation of PGCl[alpha], a chromatin immunoprecipitation
(ChIP) assay was performed according to the instructions of Upstate Biotechnology (Lake Placid, NY, USA) with minor modifications.
of in vitro translated protein from pcWNV-Cp-DJY (Cp-DJY), pcWNV-CpWT (CpWT), and pcWNVCp-3'(Cp[DELTA]3') plasmids.
They are suitable for western blotting, IHC, and immunoprecipitation
Towards this goal we have established a novel assay we named piRNA-related insertional chromatin immunoprecipitation
DNA extraction and methylated DNA immunoprecipitation
8226; RIP-Assay[TM] Kit - RNA-Binding Protein Immunoprecipitation
The new antibodies are part of the company's lipoprotein offering, also including the LipoSep IP (LS-01) Immunoprecipitation
reagent for isolation of HDL particles, as well as the Dextran Sulfate (DS109), with a 40,000 MW, which is used to identify HDL concentration by chemical precipitation.
and real-time polymerase chain reaction demonstrated that EORP markedly reduced the interaction of human antigen R protein (HuR) with the 3'-UTR of ICAM-1 mRNA in LPS-stimulated HASMCs.
Porvair Filtration Group's Chromatrap technology can develop more efficient and more precise Chromatin Immunoprecipitation
Total protein from exudates (20 jug) or 10 [micro]L of the eluted fraction from the immunoprecipitation
were denatured at 100[degrees]C for 5 min in loading buffer containing 125 mmol/L Tris (pH 6.