immunoperoxidase staining

immunoperoxidase staining

a technique of histological staining that provides morphological details and immunological identification. Analogous to immunofluorescence techniques, but uses peroxidase conjugated to immunoglobulin instead of fluorescent dyes.
References in periodicals archive ?
Identification of leishmania amastigotes in canine tissues by immunoperoxidase staining.
Immunoperoxidase Staining of COX-2 of Weil-Differentiated Carcinoma Coion, There is Intense Cytoplasmic Staining of Malignant Ceiis x100
The 2013 College of American Pathologists/International Association for the Study of Lung Cancer/Association for Molecular Pathology (CAP/IASLC/AMP) clinical practice guidelines for molecular testing of lung cancer include subtyping non-small cell lung carcinoma into adenocarcinoma and squamous cell carcinoma by morphology, by limited immunoperoxidase staining, or by both methods, as well as providing adequate material for EGFR mutational analysis and detection of ALK rearrangements.
Immunoperoxidase staining was positive for kappa, and negative for lambda, light chains.
For immunohistochemical (IHC) staining, the expression of Wilms' tumour-1 (WT-1), caspase-3 and transforming growth factor-beta 1 (TGF-b1) was detected by immunoperoxidase staining with antibodies, including rabbit monoclonal WT-1 (Santa Cruz, USA), rabbit polyclonal active-caspase-3 (Santa Cruz, USA) and rabbit polyclonal TGF-b1 (Santa Cruz, USA).
Indirect immunoperoxidase staining of tissue sections was performed, and the samples were incubated overnight with primary antibodies against Ki-67, ICAM-1, (Cambridge, MA, USA), eNOS, activated caspase-3 (Sigma-Aldrich, USA), and ROCK1 and ROCK2 (Proteintech, USA) at 4[degrees]C.
Small amounts of viral antigen were detected in a necrotic glomerulus and within the media of a renal arteriole by immunoperoxidase staining.
Comparison of Chemicon SimulFluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence staining for detection of varicella-zoster virus.
PBMC infection was confirmed by RT-PCR (figure 1A) and the presence of infectious virions in the supernatants, which were detected in LLC-MK2 cells by immunoperoxidase staining (figure 1C).
Immunoperoxidase staining for AE1/AE3, CEA and CK5/6 showed positive staining of the cyst lining for all three markers (Figure 2 A-F).
During the 1980s, antigen detection techniques, including immunoperoxidase staining, fluorescent antibody staining, and enzyme immunoassays (EIA), became widely adopted as solutions to viral culture.