immunoelectron microscopy

[im′yənō′ilek′tron]

electron microscopy of specimens labeled with antibodies that have been conjugated with gold. The gold makes the antibody labels electron-dense.

microscopy

examination with a microscope.

confocal microscopy

a technique for obtaining high resolution images and 3-D reconstructions of biological specimens; a laser light beam is expanded to make optimal use of the optics in the objective lens and is turned into a scanning beam via an x-y deflection mechanism and is focused to a small spot by the objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and after conversion into a static beam by the x-y scanner device is focused onto a photodetector (photomultiplier) via a dichroic mirror (beam splitter) to create the final image. Called also laser scanning microscopy; confocal scanning laser microscopy.

immunoelectron microscopy

the mixing of antibody with an antigen such as a virus on a specimen grid so as to increase the probability of visualizing a virus and to identify (type) the kind of virus present in the specimen, or antibody may be conjugated with gold and used to visualize and determine the location of specific antigenic determinants on a specimen.

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microscopy	CONCLUDING REMARKS It is anticipated that these simple and efficient sampling and stereological estimation procedures will contribute to future studies involving quantitative structural analysis and immunoelectron microscopy of the retina to complement those undertaken on other regions of the nervous system. Stereology and some structural correlates of retinal and ... by Mayhew, Terry M. / Image Analysis and Stereology The material covers basic methods of antigen selection and preparation, antibody production, and reagent manufacture, as well as a number of advanced protocols such as techniques for making new monoclonal cell lines, producing monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. Immunochemical Potocols, 3d ed by SciTech Book News Rapid immunologic methods that improve sensitivity when searching for unknown agents include solid-phase immunoelectron microscopy (SPIEM) (38) and serum in agar (SIA) (39), both of which may use either pooled human immunoglobulins (HuIgG) or specific antibodies. Electron microscopy for rapid diagnosis of infectious agents in ... by Gelderblom, Hans R. / Emerging Infectious Diseases	immunocomplex immunocomplex assay immunocomplex hypersensitivity immunocomplex-mediated hypersensitivity reaction immunocompromised immunocompromised host immunoconglutinin immunoconjugates immunocyte immunocytoadherence immunocytochemical staining immunocytochemistry immunodeficiency immunodeficiency disease immunodeficiency disorder immunodeficiency syndrome immunodeficient immunodepression immunodermatology immunodiagnosis immunodiagnostic immunodiffusion immunodominance immunoelectroadsorption immunoelectrodiffusion immunoelectron microscopy immunoelectrophoresis immunoenhancement immunoenhancer immunoferritin immunoferritin technique immunofiltration immunofixation immunofixation electrophoresis immunofluorescence immunofluorescence test immunofluorescent Immunofluorescent assay immunofluorescent microscopy immunofluorescent stain immunogen immunogenetics immunogenic immunogenicity immunogens immunoglobulin immunoglobulin A immunoglobulin A deficiency immunoglobulin D Immunoglobulin Deficiency Syndromes immunoglobulin E	Immunodeficiency syndromes Immunodeficiency syndromes Immunodeficiency syndromes Immunodeficiency, Centromere Instability and Facial Anomalies Syndrome immunodeficient immunodeficient immunodeficient immunodepressant immunodepressant immunodepressant immunodepression immunodepression immunodepressive immunodepressive Immunodermatology immunodiagnoses immunodiagnoses immunodiagnosis immunodiagnosis immunodiagnostic immunodiagnostic Immunodiagnostics immunodiffusion immunodiffusion immunodiffusion tests immunodiffusion tests immunodominance Immunodysregulation, Polyendocrinopathy, Enteropathy, X Linked immunoelectroadsorption immunoelectrodiffusion immunoelectron microscopy immunoelectrophoresis immunoelectrophoresis immunoelectrophoresis immunoelectrophoresis immunoenhancement immunoenhancer Immunoenzymatic Staining Technique immunoferritin immunoferritin technique immunofiltration Immunofixation Immunofixation Agarose Gel Electrophoresis Immunofixation Electrophoresis Immunofixation Electrophoresis immunofluorescence immunofluorescence immunofluorescence immunofluorescence Immunofluorescence Antibody Technique Immunofluorescence Antibody Test Immunofluorescence Assay Immunofluorescence Digital Image Processing immunofluorescence test immunofluorescence test immunofluorescent immunofluorescent immunofluorescent immunofluorescent antibody test Immunofluorescent Antibody to Membrane Antigen Immunofluorescent assay

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References in periodicals archive

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microscopy

CONCLUDING REMARKS It is anticipated that these simple and efficient sampling and stereological estimation procedures will contribute to future studies involving quantitative structural analysis and immunoelectron microscopy of the retina to complement those undertaken on other regions of the nervous system.

Stereology and some structural correlates of retinal and ... by Mayhew, Terry M. / Image Analysis and Stereology

The material covers basic methods of antigen selection and preparation, antibody production, and reagent manufacture, as well as a number of advanced protocols such as techniques for making new monoclonal cell lines, producing monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.

Immunochemical Potocols, 3d ed by SciTech Book News

Rapid immunologic methods that improve sensitivity when searching for unknown agents include solid-phase immunoelectron microscopy (SPIEM) (38) and serum in agar (SIA) (39), both of which may use either pooled human immunoglobulins (HuIgG) or specific antibodies.

Electron microscopy for rapid diagnosis of infectious agents in ... by Gelderblom, Hans R. / Emerging Infectious Diseases

immunocomplex

immunocomplex assay

immunocomplex hypersensitivity

immunocomplex-mediated hypersensitivity reaction

immunocompromised

immunocompromised host

immunocytochemical staining