immunodiffusion


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immunodiffusion

 [im″u-no-dĭ-fu´zhun]
the diffusion of antigen and antibody from separate reservoirs to form decreasing concentration gradients in hydrophilic gels.

im·mu·no·dif·fu·sion

(im'yū-nō-di-fyū'zhŭn, im-ū'nō-),
A technique to study antigen-antibody reactions by observing precipitates formed by antigen-antibody complexes, which are combinations of specific antigen and antibodies separately placed in a gel and diffused.

immunodiffusion

/im·mu·no·dif·fu·sion/ (-dĭ-fu´zhun) any technique involving diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, resulting in a precipitin reaction.

immunodiffusion

[-difyo̅o̅′zhən]
Etymology: L, immunis + diffundere, to spread
a technique for the identification and quantification of any of the immunoglobulins. It is based on the presence of a visible precipitate that results from an antigen-antibody combination under certain circumstances. Gel diffusion is a technique that involves evaluation of the precipitin reaction in a clear gel, seen when an antigen placed in a hole in the agarose diffuses evenly into the medium. An obvious ring forms where the antigen meets the antibody. Electroimmunodiffusion is a gel diffusion to which an electrical field is applied, accelerating the reaction. Double gel diffusion is a technique that permits identification of antibodies in mixed specimens. In an agar plate antigen is placed in one well, antibody in another. Antigen and antibody diffuse out of their wells. In mixed antigen specimens each antigen-antibody combination forms a separate line; observation of the location, shape, and thickness of a line permits identification and quantification of the antibody.
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Immunodiffusion

im·mu·no·dif·fu·sion

(im'yū-nō-di-fyū'zhŭn)
A technique of studying antigen-antibody reactions by observing precipitates formed by combination of specific antigen and antibodies that have diffused in a gel in which they have been separately placed.

im·mu·no·dif·fu·sion

(im'yū-nō-di-fyū'zhŭn)
A technique of studying antigen-antibody reactions by observing precipitates formed by combination of specific antigen and antibodies that have diffused in a gel in which they have been separately placed.

immunodiffusion (im´ūnōdifū´-zhən),

n a technique for the identification and quantification of an immunoglobulin.

immunodiffusion

the diffusion of antigen and antibody from separate wells, usually cut in agar, such that precipitation lines form in the agar between the wells.

radial immunodiffusion (Mancini technique)
antigen diffuses into the agar which contains specific antibody and a ring of precipitate is formed, the diameter of which is directly proportional to the concentration of the antigen and can thereby be used to quantitate the amount of antigen. A reverse radial immunodiffusion test, in which antigen is incorporated in the agar, can be used to quantitate the amount of antibody in a sample.
immunodiffusion tests
include double immunodiffusion (Ouchterloney technique) which is used in the coggins test for equine infectious anemia and single immunodiffusion (Oudin technique), as well as radial immunodiffusion.
References in periodicals archive ?
The immunodiffusion method is widely used in clinical practice and it is based on the precipitation of the anti-M and anti-H antibodies.
Because hyphal forms are rarely encountered in tissue, a correlation between complement fixation titers and immunodiffusion result patterns has not been established in the literature.
Immunodiffusion detection of Clostridium botulinum colonies.
Three major techniques are available for measuring the serologic response are enzyme immunoassay (EIA), immunodiffusion (ID), and complement fixation (CF).
For example, it can accommodate standard pour, spiral or surface inoculated plates, as well as large bioassay and Single Radial Immunodiffusion (SRD) plates.
measure inhibition zones on single radial immunodiffusion (SRD) plates, and around antibiotic disks.
Immunodiffusion test A type of precipitation reaction in which antigens and antibodies are diluted and mixed by diffusion through a gel.
Eradication of bovine leukemia virus infection in commercial dairy herds using the agar gel immunodiffusion test.
However, the only difference we could confirm was that PTB tests on the Garnet Mountains elk were done using standard agar-gel immunodiffusion during all years, whereas we also used enzyme-linked immunosorbent assay during some years (d) Number of serovars tested in Yellowstone elk, upper YNP hunter-killed elk, Gravelly-Snowcrest Mountains elk, and Garnet Mountains elk were 5, 4, 7 and 8, respectively TABLE 3.