FISH (redirected from ichthyofauna)

FISH

(flourescence in-situ hybridization) Technique used to detect small deletions or rearrangements in chromosomes.

Mentioned in: Prader-Willi Syndrome

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FISH,

abbreviation for fluorescent in situ hybridization.

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FISH

Cardiology Finnish Isradipine Study In Hypertension
Molecular medicine Fluorescent In Situ Hybridization. A method for locating a segment of DNA on a chromosome. The DNA is labeled with a fluorescent dye and hybridized to a cytological preparation of chromosomes that has been denatured to allow nucleic acid hybridization between chromosomal DNA and the probe. The site of hybridization is determined by fluorescent microscopy. FISH is a hybrid of 3 technologies: cytogenetics, fluorescence microscopy, and DNA hybridization, which is used to determine cell ploidy and detect chromosome segments by evaluating interphase—non-dividing—nuclei; in FISH, fluoresceinated chromosome probes are used for cytologic analysis and cytogenetic studies, and to detect intratumoral heterogeneity. In genetics, FISH provides a physical mapping approach to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin
DNA probes may be applied to cell preparations on a slide; if the complementary DNA sequence is present, it binds to DNA and can be detected by light microscopy; FISH labels probes nonradioactively either directly with fluorochromes, or indirectly with biotin and fluorochrome-labeled avidin, with digoxeginin and fluorochrome-labeled anti-digoxeginin, or others; the use of multiple band-pass filters allows simultaneous viewing of numerous probes for different chromosomal sequences labeled with different fluorochromes; FISH is useful in cytogenetic studies, where probes for particular chromosomes—e.g., chromosomes 13, 18, 21—or chromosomal regions—e.g., ABL and BCR genes in the Philadelphia translocation—can be used for the prenatal diagnosis of common aneuploidies or to detect early stages of lymphoproliferative disorders; FISH is as sensitive as other analytical techniques—e.g., conventional cytology and flow cytometry, used to diagnose transitional cell carcinoma of the urinary bladder
Pros FISH is simpler, less labor-intensive, and time-consuming—48 hours—than classic cytogenetics—karyotyping—2-3 weeks
Cons Only one question can be asked at a time, i.e., rather than asking ‘global issues’—e.g., what is the genetic composition of a population of cells

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hybridization [hi″brid-ĭ-za´shun]

1. the production of hybrids.

2. molecular hybridization.

fluorescent in situ hybridization (FISH) a genetic mapping technique using fluorescent tags for analysis of chromosomal aberrations and genetic abnormalities. Called also chromosome painting.

molecular hybridization in molecular biology, formation of a partially or wholly complementary nucleic acid duplex by association of single strands, usually between DNA and RNA strands or previously unassociated DNA strands, but also between RNA strands; used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands.

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fish

members of the classes Cephalochordata (lancelets), Agnatha (hagfish and lampreys), Elasmobranchii (sharks and rays), Holocephali (ghost sharks), Osteichthyes (bony fish), Gastropoda (gastropods), Pelecypoda (bivalves), Cephalopoda (cephalopods), Crustacea (crustaceans).

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fish handler's disease

erysipeloid.

fish liver oils

used in animal diets because of their high content of vitamin A and D. Should be stabilized to avoid loss of vitamins in storage and need an antioxidant to avoid rancidification and loss of vitamin E. May also cause tainting of animal foods. See also cod liver oil, omega-3 fatty acids.

fish meal

a protein feed supplement rich in calcium, phosphorus and having a good iodine content. Made from inedible fish residues from the canning and fresh fish industries. May taint animal products. Toxic amines produced by bacterial spoilage cause gizzard erosion and fatal hemorrhage in birds.

fish mouth

used to describe gaping wounds of the skin.

fish mouthing

a surgical technique for anastomosing two pieces of bowel when one is moderately larger in diameter than the other. The smaller diameter is made wider by slitting it longitudinally down the sides so that it opens like a fish's mouth.

fish poisoning

see diodontidae, tetraodontidae.

fish scale disease, fish skin disease

see inherited congenital ichthyosis.

fish solubles

dehydrated fishwater from oil extraction and fishmeal industries.

fish tuberculosis

disease of aquarium fish caused by Mycobacterium spp. Causes weight loss, exophthalmos, cutaneous ulcers and pallor. At necropsy there are internal granulomas. The acid-fast organisms can be found in the ulcers. Also found in a variety of cultured species including shrimps.

fish viruses

includes rhabdoviruses and birnavirus.

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FISH

Fluorescent in situ hybridization Molecular medicine A hybrid of 3 technologies: cytogenetics, fluorescence microscopy, and DNA hybridization, which is used to determine cell ploidy and detect chromosome segments by evaluating interphase–non-dividing nuclei; in FISH, fluoresceinated chromosome probes are used for cytologic analysis and cytogenetic studies, and to detect intratumoral heterogeneity. See Chromosomal paint box.

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Patient discussion about FISH.

Q. Can omega-3 make my cholesterol lower? My doctor told me that I have too much cholesterol in my blood, and that it can cause heart attack or stroke, but if I keep it low than my risk will be lower. He told me that because it’s not that high level I can try to change my diet before I have to start taking medication. I heard that omega-3 can make my cholesterol level low. Is that true? Do I have to take it in pills? Is it safe?

A. You can consume omega-3 either as pills or in fish oil, fatty fish (such as salmon) and vegetarian food such as flaxseed and flaxseed oil, canola oil, soybean oil, and nuts.

Q. Does fish-oil helps exercise induced asthma? I was diagnosed with exercise induced asthma a couple of year ago, and since then had better and worse times with my asthma, although the treatment I get. I read in a newspaper that fish oil can help exercise induced asthma- is that true? Do I have to eat fish-oil specifically or can I eat fish instead (I really, really, hate fish-oil…)?

A. No one really proved that omega-3 actually helps asthma, although some doctors say it is. If I was in your situation, I’d just give it a try and see if it helps me (just ask your doctor before if omega 3 wouldn’t interfere with your other medications). Just remember that it’s not a magic, so don’t get disappointed if it doesn’t produce miracles on your asthma. Good luck!

Q. What's the best tropical fish food available in the market, in terms of nutrition, palatability, etc., My teenage son is not much interested in non vegetarian items except some fishes. As he is very lean and weak we like to improve his health using his favorite dish: ‘fish’. Can anyone please let me know what's the best tropical fish food available in the market, in terms of nutrition, palatability, etc.,

A. fish as a nutritional value dish is holding up - protein, iodine and omega-3 fatty acids. about what fish have the best quantities?
as GeraldRoger said- salmon fish. but that is also depends on how it was grown and where, not every fish has the same amount of everything.