The membrane integrity of sperm was evaluated by hypo-osmotic
swelling test (HOST; Revell and Mrode, 1994; Buckett et al.
Spermatozoa plasma membrane integrity: The hypo-osmotic
swelling test (HOST) solution (150 mOsm/L) was prepared by dissolving 7.
Briefly, 10 [micro]L of semen was incubated in 100 [micro]L of hypo-osmotic
solution at 37[degrees]C for 30 min.
Similarly, Liu and Foote (1998) noted higher spermatozoa motility in cattle bull semen at 300 mOsm/kg compared to hypo-osmotic
tris extender i.
La prueba hipoosmotica, reconocida como HOST por su denominacion en ingles hypo-osmotic
swelling test, es una prueba seminal simple y de bajo costo, que permite evaluar la integridad funcional de la membrana plasmatica del espermatozoide (Jeyendran et al, 1984).
In freshwater fish species, mature spermatozoa require a hypo-osmotic
shock to trigger sperm motility.
lubricants such as FemGlide and Slippery Stuff can reduce mucus production.
A physiological function of LmAQP1 was shown to be in volume regulation in response to hypo-osmotic
The relative membrane reserve of cells was evaluated in tests with hypo-osmotic
stress in vitro, according to the procedure published in earlier studies .
swelling (HOS) assay was performed as described by Jeyendran et al.
To evaluate plasma membrane integrity, two simple basic test are used: supravital staining (eosin/nigrosin) and hypo-osmotic
sweelling test (HOST/HOS) assay .
There was also a significant reduction in sperm viability and hypo-osmotic
swelling in the Ap-treated and N-9 treated groups in comparison to the control.