Biochemical basic of resistance to hygromycin B
in Streptomyces hygroscopicus-the producing organism.
Kryptolebias marmoratus embryos were tested for susceptibility to hygromycin B under various concentrations prior to the late blastula stage of development.
Keywords: Kryptolebias marmoratus, development, hygromycin B, toxicity, midblastula transition
Efficient soybean transformation using hygromycin B
selection in the cotyledonary-node method.
Although past studies have succeeded in transferring the neomycin drug resistance gene (NeoR) within cells, no studies have been conducted with the hygromycin B
drug resistance gene (HygroR) or both HygroR and NeoR.
This vector has a cytomegalovirus immediate-early promoter upstream of the multiple cloning site and a hygromycin B
resistance gene driven by an SV40 early promoter.
According to a recent purity survey, several batches of commercially available hygromycin B obtained from multinational life science reagent companies showed that the purities ranged anywhere from an alarming 60% to a high of 92%.
We ourselves were frustrated by inconsistent hygromycin B selection results.
Compared to the ordinary method in resistant analysis, this method is simpler and cheaper for using hygromycin B.
Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.
1996) reported selection of large quantities of embryogenic calli from rice seeds as target tissues and use of hygromycin B as an effective selection agent to produce fertile transgenic rice plants in the model indica variety TN 1.
1] hygromycin B (Boehringer Mannheim) CI-H50 CI with 50 mg [L.