In the liver homogenate
, the levels were raised with a high statistical significance (P<0.
The protein concentration was determined by bicinchoninic acid protein assay kit (Nanjing Jiancheng Bioengineering Institute, China), and then the homogenates
were adjusted to the same protein concentration, finally, each homogenate
was mixed with an equal amount of 2xstandard sample loading buffer and heated in boiling water for 5 min and cooled on ice for subsequent Western blot analysis.
Reextraction and testing of the original tick homogenates
using both primer/probe sets NP1 and PB1 confirmed BRBV RNA in all 3 pools.
417, respectively); and the same trend could be found for 5-HT of the brain homogenate
between GFS treatment subgroups of MI, depression, MI + depression and control groups ( P = 0.
1 mM was added to 100 [micro]g of adipocyte homogenate
Univariate PERMANOVA for the richness of bacterial colony types from the homogenate
samples revealed that on average, significantly more morphologically distinct types of bacteria were cultured on marine agar (mean 1.
induced a non-significant decrease in MDA level in jejunum, while the MLE at a dose of 800 mg/kg, caused a highly significant reduction in kidney, testis and spleen tissue homogenates
Statistical analysis of feeding behaviors in krill homogenate
plumes showed that plume type and animal were significant factors in explaining behavior; the three best-fitting models according to AIC involved these factors in various combinations (Table 1).
In comparison, incubation with mouse feces homogenate
did hardly change the crocin pattern (Table 1C) or led to the formation of significant amounts of trans-crocetin.
of whole body of Drosophila melanogaster was prepared with 50 flies in 500 [micro]L PBS, using a glass-glass grinder (PYREX 2 mL; Thermo Disher Scientific Inc.
The degree of lipid peroxidation in the homogenate
was determined by adapting the protocol described by Okhawa et al.
(50 [alpha]l) was incubated for 15 min at 41[degrees]C in a reaction mixture containing 100 [alpha]l of 160 mM Tris-HCl pH 7.