homoduplex


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homoduplex

a DUPLEX DNA in which the two strands show COMPLEMENTARY BASE PAIRING throughout. Compare HETERODUPLEX.
References in periodicals archive ?
Quantitatively, the number of possible hybrids will depend on the number of original homoduplex molecules present in the mixture.
Homoduplex and hetroduplex polymorphisms of the amplified ribosomal 16S-23S internal transcribed spacers describe genetic relationships in the "Bacillus cereus Group".
Durante la reaccion de denaturacion y renaturacion de estos grupos, se forman dos tipos de moleculas, los homoduplex y los heteroduplex.
If the sequence of the two templates is different, this procedure produces a multiple band phenotype on a denaturing gradient gel because heteroduplex DNA denatures under more stringent conditions than homoduplex DNA.
Detection of clarithromycin-resistant Helicobacter pylori strains by a preferential homoduplex formation assay.
Conformation-sensitive capillary electrophoresis (CSCE) [8] is a simple method for indirect mutation scanning that is based on the principle that homoduplex and heteroduplex DNA have different electrophoretic mobilities under partially denaturing conditions (19-21).
This method takes advantage of the dramatic drop in PCR amplification that occurs for perfectly matched homoduplex templates when the denaturation temperature is less than the critical denaturation temperature ([T.
As mentioned above, it is possible that high-abundance mutants may become less differentiated from the wild type if enrichment is quite strong and if their homoduplex melting curves are comparable to the wild-type melting curve (4).
The altered melting curve shape is a result of the presence of both heteroduplex and homoduplex amplicons in the PCR product, vs only homoduplexes from normal samples.
If no SNP is present, all of the homoduplex DNA fragments elute as a single peak on the chromatogram.
Different homozygotes each produce only a single homoduplex species.