high-pressure liquid chromatography


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high-per·for·mance liq·uid chro·ma·tog·ra·phy (HPLC),

a chromatographic technology used to separate and quantitate mixtures of substances in solution. A sample is injected into a moving stream of solvent that flows through a column and detector. Separation during passage through the column occurs by absorption, partition, ion exchange, or size exclusion. The technique is commonly used in laboratories to measure organic compounds including steroid hormones, pesticides and poisons, toxic and carcinogenic compounds, and drugs.

high-pressure liquid chromatography

a method of chromatography for separating and quantitating mixtures of substances in a solution. The procedure uses a high-resolution column, pressure, and gradient elution systems for separation of solutes by absorption, partition, ion exchange, and size exclusion. Also called high-performance liquid chromatography.

high-per·for·mance liq·uid chro·ma·tog·ra·phy

(HPLC) (hī pĕr-fōr'măns lik'wid krō'mă-tog'ră-fē)
A chromatographic technology used to separate and quantitate mixtures of substances in solution. The technique is used to measure organic compounds, including steroid hormones, pesticides and poisons, toxic and carcinogenic compounds, and drugs.
Synonym(s): high-pressure liquid chromatography.
References in periodicals archive ?
Postmortem femoral blood samples were analyzed for NSAIDs using high-pressure liquid chromatography, and specimens of gastric and duodenal mucosa were examined for coexisting pathologic conditions.
First, Daly separated the alkaline components from one another using high-pressure liquid chromatography.
Chitwood's test, which was developed at the ARS Nematology Laboratory in Beltsville, Maryland, is a modification of a method called reversed-phase high-pressure liquid chromatography.
The researchers bought 10 commercially available thyroid supplements from stores or websites and used high-pressure liquid chromatography to separate and identify the chemical components of T3 and T4.
Chromatographic system for high-pressure liquid chromatography with a diode-matrix UV detector
Epidermal retinoid and beta-carotene concentrations were determined by high-pressure liquid chromatography.
Currently, four different techniques are routinely used in the lab setting: l) alkaline and acid gel electrophoresis: 2) isoelectric focusing (IEF); 3) high-pressure liquid chromatography (HPLC); and 4) capillary electrophoresis (CE).
However, a biologic marker of Pb exposure, [delta]-aminolevulinic acid ([delta]-ALA) may be detected in urine, plasma, and serum using high-pressure liquid chromatography with fluorescence detection.
These are followed by a high-pressure liquid chromatography post-column reaction of the extracted material.
High-pressure liquid chromatography revealed two or perhaps three proteins within the sample, they report.
Androgenic activity of chemicals in the medium was detected using an androgen receptor transcription assay and was quantified by high-pressure liquid chromatography (HPLC).
High-pressure liquid chromatography procedures are being utilized to improve the separation and quantification of the amines.

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