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hexamer

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hex·a·mer (hks-mr)
n.
1. A polymer molecule composed of six monomers.
2. A capsomer with six structural subunits.


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cDNA reactions consisted of 2 [micro]L of extracted viral RNA, 50 ng of random hexamers (Applied Biosystems, Foster City, CA, USA), 4 [micro]L of 5x first strand buffer (Invitrogen, Carlsbad, CA, USA), 200 [micro]mol/L of each dNTP (GE Healthcare, Piscataway, NJ, USA), 40 U of RNasin ribonuclease inhibitor (Promega, Madison, WI, USA), and 200 U of SuperScript II reverse transcriptase (Invitrogen) in a total volume of 20 [micro]L.
Functional effects were considered possible if substitutions were predicted to affect the active site, access to the active site via solvent channels, sites of interaction between monomers involved in forming the active hexamer and/or overall protein stability.
 
 
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