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hexamer |
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In accordance with the Invitrogen manual, eDNA was synthesized, by using random hexamers (Takara Bio Inc. Reverse transcription was carried out by incubating 2 tag total RNA with 5 mM random hexamer oligonucleotides, 10 mM DTT, 2. They assigned each pentamer to occupy 72 percent as much area as each hexamer did. |
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