Children with acute infectious mononucleosis were diagnosed by symptoms, >10% atypical lymphocytes, and a positive heterophile antibody
It is correct, that for the purposes of the algorithm, we have assumed that all persons with a positive heterophile antibody
test had infectious mononucleosis and did not need to be routinely evaluated further virologically.
In addition, a control group that consisted of a selection of 50 patients from the 1689 original patients who were heterophile antibody
negative but who had a normal lymphocyte count and generated no atypical lymphocyte flag were also tested for EBV IgM antibodies.
To investigate whether heterophile antibody
interference was a cause for the discrepant results, we incubated 11 consecutive discrepant samples (between September 7, 2011, and September 15, 2011) in Scantibodies HBT tubes.
Although the heterophile antibody
test may occasionally be false positive in patients with autoimmune diseases, serum sickness, pregnancy, or HIV infection, false negatives are far more frequent (about 15% overall), particularly in children who are under 4 years of age (approximately 45%).
56%) were classified as outliers, and no heterophile antibody
interference was identified with this assay.
As illustrated in this clinical case, heterophile antibody
blocking tubes can be used to eliminate the interference, although this approach does not always eliminate the interference.
On the other hand, the absence of such a TSH increase in the patient as recently as 4 months earlier suggested no heterophile antibody
binding to the variable region of the assay antibody (idiotypic interaction) is less common (5).
1) published in this issue of Clinical Chemistry describes the clinical work-up of a patient with low-positive human chorionic gonatodtropin (hCG) due to pituitary or heterophile antibody
Care has been taken to block such activities at heterophile antibody
titers commonly encountered in a population, but rare samples with higher titers may affect the test.
Phantoms in the assay tube: heterophile antibody
interferences in serum thyroglobulin assay.