hanging drop preparation

hanging drop preparation

Etymology: ME, hangen, to hang; AS, dropa, to fall; L, praeparer, to make ready
a technique used for the examination and identification of certain microorganisms, such as spirochetes or trichomonads. The technique requires a coverslip, a microscope, and a special slide that has a central concavity. A specimen suspected of containing the microorganism is diluted with a sterile isotonic solution. A drop of this fluid mixture is placed on a glass coverslip, which is then inverted carefully and placed over the slide so that the drop is hanging from the slip into the concavity in the slide. The delicate structures and the method of movement characteristic of the species may then be viewed through the microscope.
References in periodicals archive ?
The most rapid presumptive method to identify the presence of vibrio, in the stool sample is to observe for darting type of motility in the hanging drop preparation of the stool; but culture is must as culture consider as gold standard method for identification.
Stool microscopy and hanging drop preparation examination were unremarkable.
Colonies obtained were stained and hanging drop preparation for motility was done.