Summary: TEHRAN (FNA)- Incorporating guanidinium
into perovskite solar cells stabilizes their efficiency at 19 percent for 1,000 hours under full-sunlight testing conditions, report scientists.
Single-step method of RNA isolation by acid guanidinium
DNA was extracted from the samples in guanidinium
buffer (Pochon et al.
Extracts were prepared using commercially available, off the shelf, preformatted, guanidinium
thiocyanate based total nucleic acid purification kits following the manufacturer's instructions (QIAGEN, Valencia, California).
Single Step Method of RNA Isolation by Acid Guanidinium
Nucleic acid extraction is often carried out with commercial guanidinium
Samples were previously homogenized in a tissue homogenizer (FastPrep 5G, MP Biomedicals, USA) using plastic vials containing zirconium spheres (Lysing matrix A; MP Biomedicals, USA); after that, the guanidinium
thiocyanate-phenol-chloroform method was used for the extraction (Chomczynski & Sacchi, 2006).
isothiocyanate (GIT) extraction of nucleic acids was performed on all samples (Turner et al.
Sacchi, "Single-step method of RNA isolation by acid guanidinium
thiocyanate-phenol-chloroform extraction," Analytical Biochemistry, vol.
With slight modifications, total RNA was purified from 150-250 mg backfat and muscle tissues by the guanidinium
thiocyanate-phenol-chloroform extraction method as described by Chomczynski and Sacchi (1987), using TRI Reagent Solution (Life Technologies) and 1-bromo-3-chloropropane (VWR International), and rehydrated in DEPC-treated water.
Total RNA extracted from mock- or drug-treated cells was isolated using the guanidinium
thiocyanate method (Trizol; Invitrogen) following the manufacturer's protocol.
Afonso, Comparison of Physicochemical Properties of New Ionic Liquids Based on Imidazolium, Quaternary Ammonium and Guanidinium