Total RNA extracted from mock- or drug-treated cells was isolated using the guanidinium
thiocyanate method (Trizol; Invitrogen) following the manufacturer's protocol.
Single-step method of RNA isolation by acid guanidinium
For isolation of total RNA from mouse liver, we used a guanidinium
thiocyanate/phenol/chloroform method with TRIzol reagent (Life Technologies) according to the manufacturer's protocol.
For clarity, an example of a solution-phase method is the conventional process based on guanidinium
thiocyanate-phenol-chloroform, while silica is an example of a solid-phase medium used in the isolation of nucleic acids.
They do this by modifying nucleotides with imidazole, ammonium and guanidinium
groups similar to those found in the protein.
The tensile strength measurements were conducted with ethnic hair which had been straightened by guanidinium
The increased acceleration activity of guanidine carbonate is believed to be due to the presence of carbon-nitrogen double bond (C=N) containing singly charged guanidinium
We prepare our own RNA from mouse intestinal tissue, using guanidinium
isothiocyanate (Chomczynski & Sacchi, 2006).
Single step RNA isolation by acid guanidinium
thiocyanate phenol chloroform extraction.
Novel use of guanidinium
isothiocyanate in the isolation of Mycobacterium tuberculosis DNA from clinical material.
On the ends of the chains, Mynar created branches tipped with guanidinium
, a chemical relative of the amino acid arginine.
About 5,000 insects were ground in liquid nitrogen and total RNA was extracted with guanidinium
salt-phenol-chloroform procedure as previously described by Strommer et al.