was performed with (A) 80% methanol and (B) 20% water (pH 3.
The fractionation of the ethyl acetate extract was done through a series of gradient elution
normal phase gravity column chromatography (GCC) on a 60 - 230 mesh silica gel stationary phase .
The described method of the prediction of retention time can substantially reduce the time required to find optimal conditions in gradient elution
6 x 250 mm, 5 [micro]m; Agilent) with gradient elution
(at the rate of 1.
Contract notice: Supply liquid chromatograph with gradient elution
, equipped with refractive index detector, uv detector, automatic injection of sample, communication with different parts
The combination of low gradient delay volume and high precision gradient formation was useful for high-throughput gradient elution
8 mL/min according to a linear gradient elution
program: initial 50% B; 0.
1 x 150 mm) was connected to the XTerra column and the following gradient elution
profile was used to elute any organic molecules that were adsorbed on the XTerra column: 100 to 0% water for 30 mins, hold at 0% water for 10 mins.
In other attempts SK was fractionated by ammonium sulfate and further purified by gradient elution
from a DEAE-cellulose chromatography column (17).
Table 1: Gradient elution
method performed with binary solvent system using as mobile phase.
Solvents were delivered according the following program: linear gradient elution
from 10% A and 90% B to 85% A and 15% B within 18 min; 85% A and 15% B for 5 min; linear gradient elution
from 85% A and 15% B to 10% A and 90% B within 7 min.
A gradient elution
was carried out at a flow rate of 1.