Bruce Burnett, the Principal Investigator of the NIH grant said, "Given that immortalized and non-transformed gonadotropes
can be developed, Vitro intends to use these cells for basic research and for discovery of new drugs for treatment of infertility.
The pulsatile secretion of gonadotrophin releasing hormone (GnRH) activates the pituitary gonadotropes
and leads to the release of the gonadotrophins .
James Musick, Vitro's President and CEO said, "Since moving our operations to the Fitzsimons Bioscience Center in June 2001, a Colorado biotechnology hub located in the metro Denver area, we have experienced significant advances in our efforts to immortalize human gonadotropes
, the cells of the pituitary gland that produce FSH and LH.
The objective of the present study was to determine whether exposure of pregnant sheep to environmental concentrations of a mixture of EDCs, via sewage sludge used as a fertilizer, disrupts the kisspeptin/GPR54 system, as determined by examination of KiSS-1, GPR54, and ER[alpha] (estrogen receptor-[alpha]) mRNA expression in the adult and fetal hypothalamus and pituitary and the proportions of kisspeptin positive LH-secretory gonadotropes and estradiol-receptive gonadotropes within the fetal pituitary gland.
05) reduction in the number of LH[beta] immunopositive cells in the fetal pituitary gland (Figure 3G) and a significant reduction in the number of cells double labeled for LH[beta] and kisspeptin (expressed as the number of LH[beta] positive gonadotropes per field; p < 0.
The results of the present study also indicate that maternal EDC exposure reduces fetal pituitary KiSS-1 mRNA expression as well as lowering the proportion of gonadotropes that coexpressed kisspeptin and LH[beta] and the number of LH[beta] positive gonadotropes.
Because 92% of ER[alpha]-positive cells are also LH[beta]-positive gonadotropes, this reflects a significant disturbance of the interactions among kisspeptin, estrogens, and LH by chemical/EDC exposure in utero.
Signal transduction mechanisms mediating secretion in goldfish gonadotropes
Many degenerating and nondegenerated gonadotropes, somatotropes, and lactotropes showed dilated ER (Figures 3 and 5) and hypertrophic Golgi areas, occasionally with some lightly dense granules inside.
In addition, the transient but dramatic decrease in the circulating level of testosterone may stimulate cellular activities of gonadotropes.
Sutton H6, Fusco A, Cornwall GA, Cystatin-related epididymal spermatogenic protein colocalizes with luteinizlng hormone-beta protein in mouse anterior pituitary gonadotropes