To a solution of (10 mmol) Glycylglycine
in 10 mL of Methanol, KOH (10 mmol) pellets were dissolved by continuous stirring in a 100 mL Round Bottom flask.
alkaline phosphatase kinetic p-Nitrophenyl phosphate buffer and AMP, according to the IFCC, AST kinetic, according to IFCC without pyridoxal phosphate, ALT kinetic, according to IFCC without pyridoxal phosphate, bilirubin direct from vanadate, sodium, total bilirubin with vanadate, sodium, calcium total of arsenazo (III), total cholesterol esterase, and the enzyme cholesterol oxidase, cholesterol direct HDL, LDL cholesterol direct-, creatinine enzymatic GGT gamma-glutamyl-3-carboxy-p-nitroanilide and glycylglycine
, by IFCC, glucose with the enzyme hexokinase
Effect of hydrogen ion-gradient on carrier-mediated transport of glycylglycine
across brush border membrane vesicles from rabbit small intestine.
The activity of serum GGT was measured according to Szasz in which the substrate L-[gamma]-glutamyl-3-carboxy-4-nitroanilide in the presence of glycylglycine
is converted by GGT in the sample to 5-amino-2-nitrobenzoate which can be measured at 405 nm.
Glycine, alanine, serine, threonine, asparagine, proline, methionine, glutamic acid, tyrosine, aspartic acid, tryptophan, lysine hydrochloride, arginine hydrochloride, ethylene glycol, glycylglycine (G1yGly), glycylglycylglycine (G1yGlyGly),hexaglycine [[(Gly).
The absorbance maximum shifted from ~665 nm for reagent alone to 640-650 nm for dipeptides such as glycylglycine, 620-640 nm for amino acids such as threonine, asparagines, histidine, and glycinamide, ~600-610 run for tripeptides, and 560-610 run for longer peptides.
When glycine is combined with another amino acid, the combining form is GLYCYL-, so when two glycines are combined, the result is called GLYCYLGLYCINE
hydrochloride (Sigma), sodium chloride UT Baker), sodium bicarbonate UT Baker), potassium chloride (Merck), urea (Merck), ammonium chloride UT Baker), vitamin C (Aldrich), citric acid (Aldrich), lactic acid sodium salt (Fluka), pyruvic acid sodium salt (Sigma-Aldrich), chicken egg albumin (Sigma-Aldrich), bovine [gamma]-globulins from Cohn fraction II and III (Sigma), chicken egg white lysozyme (Sigma-Aldrich), 2,2-diethoxyacetophenone (DEAP; Acros Organics), acrylamide (AA; Sigma), NN'-methylenebisacrylamide (bisAA; Sigma), poly(ethylene glycol), monomethacrylate 400 (PEG; Polysciences, Inc.
Table 4 Analysis of Line weaver-Burk plot of Glycylglycine
dipeptidase in Rat jejunum.
A successful assay was eventually established that utilizes the ability of ACE to hydrolyze the tripeptide N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine to furylacryloylphenylalanine and glycylglycine
Because L-lactate preparations can be contaminated with the D-isomer, we proceeded as follows: A solution of 50 mmol/L L-lactate was prepared in glycylglycine
and glutamate buffer, to which we added [NAD.