The confirmed Candida isolates were further speciated by germ tube test
, chlamydoconidia production test on corn meal agar, sugar assimilation tests for Dextrose, Lactose, Sucrose, Trehalose and Maltose and by the colour of growth on HiChrom Candida agar isolation method, Hi media laboratories Pvt.
Identification was carried out by performing Gram stain, germ tube test
Yeast isolates were further analysed through the germ tube test
and reported as candida albicans or candida non-albicans.
The Candida isolates obtained were further identified by conventional methods such as gram stain (direct microscopy), germ tube test
, Sabouraud dextrose broth, microscopic morphology on cornmeal agar, and sugar assimilation tests.
Germ tube test
was performed to identify Candida albicans.
Candida species, initially identified by colony morphology, were further characterized by germ tube test
, morphology on cornmeal as well as Chromagar and sugar assimilation profiles.
Until then it had been misidentified as candida albicans if only germ tube test
was used for identification as both the species have many similarities in their microscopic morphology and ability to form germ tubes in serum.
All consecutive non-duplicate Candida species isolated from March to September 2005 were identified using the germ tube test
and a commercial identification kit (Auxacolor 2, Bio-Rad, France).
In addition to variation in trehalose assimilation rates, 3-4 distinct types were apparent in the germ tube test
The isolate was confirmed to be C albicans by the germ tube test
and the API 20 C AUX kit (biomerieux, Marcy-L'Etoile, France).
These colonies were further followed by performing germ tube test
, morphology on corn meal agar, sugar fermentation, and assimilation test for identification of Candida species through conventional method.
To confirm presence of candida gram staining and germ tube test
were also carried out.