ascription by RFLP analysis of the 16S rRNA gene grouped all the isolates under genomovar
In order to develop rapid tests to determine the genomovar
status of BCC isolates, nucleotide sequence polymorphism in two genes, the 16S rRNA gene (16S rDNA) and recA gene has been examined, and only recA based typing has been found useful to identify BCC strains from both environmental samples and sputum as well as to identify other Burkholderia species.
We are looking forward to using PCR technology in many more areas, including virus detection, unculturable agents, rapid detection of agents of septicemia and of bioterrorism, antibiotic resistance and genomovar
Strains being developed for agricultural use seem to cluster in genomovar
Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar
Evaluation of species-specific recA-based PCR tests for genomovar
level identification within the Burkholderia cepacia complex.
abscessus isolates belonged to genomovars
T, -5a, and -6, with a slightly higher prevalence of genomovar
Endemicity and inter-city spread of Burkholderia cepacia genomovar
III in cystic fibrosis.
5%) were from genomovar
III and included all strains that clustered in individual centers and appeared to be transmitted from patient to patient.
cepacia complex were recovered from 447 patients in 8 of the 10 provinces; 369 (83%) of these patients had genomovar
III and 43 (9.
We did not include these divergent genomovars
(genetic lineages) in subsequent SNP and phylogenetic analyses.
At least five genomovars
(discrete species) constitute what has recently been designated the "B.