gel filtration


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gel fil·tra·tion

separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range; the larger the molecule, the less time it spends in the interior of the beads, thus emerging earlier from the column than smaller molecules.

gel filtration

a method of separating molecules by size. A solution containing molecules of various sizes is passed through a filter consisting of a porous material, generally a polyacrylamide or polysaccharide. The larger molecules are excluded from the interior of the filter and thus emerge from it earlier than the smaller molecules.

gel fil·tra·tion

(jel fil-trāshŭn)
Separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range.
References in periodicals archive ?
5] Nonstandard abbreviations: TnI, troponin I; TnT, troponin T; TnC, human cardiac/slow skeletal troponin C; cTnI, human cardiactroponin I; cTnT, human cardiactroponinT; AMI, acute myocardial infarction; TnAAbs, autoantibodies specific to human cardiac troponins; I-T-C, human cardiac troponin ternary complex; aar, amino acid residues; I-C, human cardiac troponin binary complex; mAb, monoclonal antibody; ECG, electrocardiogram; GF, gel filtration.
The gel filtration methods are based on the principle that different molecules can be separated on the basis of differences in their size by passing them through a column containing swollen particles of a gel.
In the gel filtration chromatography, the maximum activity of Reutericin_LHS was observed in the fractions (32-38).
Purification strategies used for bacterial lipase purification Pseudomonas spp Purification Source strategies Pseudomonas Ion exchange Lee and Rhee (1993) putida 3SK chromatography and gel filtration Pseudomonas Ammonium sulfate Sharon et al.
Table 3: Comparison of four fractions from gel filtration of n-Hexane crude leaf extract on the mortality (LT50 in h) of termites
In the present study, gel filtration chromatography was carried out in order to obtain highly purified M13 phages from the supernatant of the inoculated E.
1999, "Purification of antibodies using ammonium sulfate fractionation or gel filtration," Meth.
The molecular masses of these fractions were estimated by gel filtration chromatography (Table 1).
Vaccine research evaluates the potential of dendritic cells, T27K fractionated by gel filtration and anion exchange, and a recombinant Ag2/PRA106 + CSA chimeric fusion protein.
Purification was done by ion exchange chromatography using Q sepharose followed by gel filtration chromatography using Sephacryl S300.
Known volumes of low-speed supernatants and fractions from gel filtration were added to equal volumes of 2X sample buffer (Laemmli, 1970) and boiled for 5 min.