gel filtration


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gel fil·tra·tion

separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range; the larger the molecule, the less time it spends in the interior of the beads, thus emerging earlier from the column than smaller molecules.

gel filtration

a method of separating molecules by size. A solution containing molecules of various sizes is passed through a filter consisting of a porous material, generally a polyacrylamide or polysaccharide. The larger molecules are excluded from the interior of the filter and thus emerge from it earlier than the smaller molecules.

gel fil·tra·tion

(jel fil-trāshŭn)
Separation of molecular sizes by passage of a mixture through columns of beads of cross-linked dextrans or similar relatively inert material of a well-defined pore size range.
References in periodicals archive ?
Using the same gel filtration technique (16,17), we observed that this type of GFC column was not suitable for adequate separation of the ternary-bound cardiac troponin T-I-C complex ([V.
1999, "Purification of antibodies using ammonium sulfate fractionation or gel filtration," Meth.
Alpha amylase was purified from culture supernatant by using ammonium per sulfate, sepharose gel filtration and DEAE cellulose chromatography.
Gel filtration chromatography: Sephacryl--S 300 (Pharmacia, Sweden) was used for gel filtration chromatography.
Single agarose gel beads of various concentration (mainly 2, 4, and 6% of the agarose content) were tested for use in gel filtration techniques.
of Chromatography (23) (it became one the twenty most cited papers in the 500 first volumes of the journal), in my PhD thesis (24), and in the Pharmacia booklets on Gel Filtration {1959).
In figure 1 the result of gel filtration according to molecular weight is showed.
com/research/df919c/modern_sizeexclus) has announced the addition of John Wiley and Sons Ltd's new report "Modern Size-Exclusion Liquid Chromatography : Practice of Gel Permeation and Gel Filtration Chromatography, 2nd Edition" to their offering.
Further steps comprised anion exchange, immunoaffinity and gel filtration chromatographies.