fura-2


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fu·ra-2

(fū'ră),
A fluorescent indicator that binds calcium; it is excited at longer wavelengths when free of calcium than when calcium is bound; the ratio of fluorescence intensity at two excitation wavelengths provides a measure of free calcium ion concentration; may be injected into cells to monitor moment-to-moment changes in intracellular free calcium ion concentration.
See also: aequorin.

fu·ra-2

(fūr'ă)
A fluorescent indicator that binds calcium; it is more excited at longer wavelengths when free of calcium than when calcium is bound; the ratio of fluorescence intensity at two excitation wavelengths provides a measure of free calcium ion concentration; may be injected into cells to monitor moment-to-moment changes in intracellular free calcium ion concentration.
See also: aequorin
References in periodicals archive ?
2+] imaging of BEAS-2B cells was conducted using 2 [micro]M fura-2 acetoxymethyl ester for loading and following a protocol for ratio-metric [Ca.
2+]-sensitive fluorescent dye Fura-2 aceto-oxymethyl ester (Fura-2/AM, Dojindo, Japan) as previously described (17,18).
The behavior of cytosolic calcium mobilization revealed by fura-2 closely resembled that of elastase, thus suggesting that they may be related.
Intracellular [Ca2+]i was determined by assaying for levels of fura-2, a [[Ca.
Isolated smooth muscle cells were loaded with Fura-2 AM, a fluorescent dye that binds to diatomic ions and has a high affinity for [Ca.
This conclusion is supported by preceding studies with two calcium probes, Indo-1 and Fura-2, which also detected no difference [11, 12].
1988) used microinjected fura-2 combined with image analysis to determine [Ca.
Calcium mobilization in MDA-MB-231 cells was performed using a Fura-2 calcium assay kit according to the manufacture's protocol (Invitrogen).
0 [micro]M Fura-2 AM (Dojindo, Japan) in HBSS for one hour at room temperature (24 to 26[degrees]C) and washed with HBSS.