fura-2


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fu·ra-2

(fū'ră),
A fluorescent indicator that binds calcium; it is excited at longer wavelengths when free of calcium than when calcium is bound; the ratio of fluorescence intensity at two excitation wavelengths provides a measure of free calcium ion concentration; may be injected into cells to monitor moment-to-moment changes in intracellular free calcium ion concentration.
See also: aequorin.

fu·ra-2

(fūr'ă)
A fluorescent indicator that binds calcium; it is more excited at longer wavelengths when free of calcium than when calcium is bound; the ratio of fluorescence intensity at two excitation wavelengths provides a measure of free calcium ion concentration; may be injected into cells to monitor moment-to-moment changes in intracellular free calcium ion concentration.
See also: aequorin
References in periodicals archive ?
Fura-2 AM and BAPTA-AM were obtained from Dojindo Molecular Technologies, Inc.
2+]-sensitive fluorescent dye Fura-2 aceto-oxymethyl ester (Fura-2/AM, Dojindo, Japan) as previously described (17,18).
The behavior of cytosolic calcium mobilization revealed by fura-2 closely resembled that of elastase, thus suggesting that they may be related.
2+] measurement using fura-2 dye in aortic strips revealed that arsenite enhanced vasoconstriction induced by high [K.
This conclusion is supported by preceding studies with two calcium probes, Indo-1 and Fura-2, which also detected no difference [11, 12].
At present, the most widely used chemical probe is FURA-2, which changes its fluorescent emission as it binds to free calcium ions.
Intracytoplasmic esterases hydrolyze Fura-2/AM to Fura-2 that, in turn, binds intracytoplasmic free [Ca.
Drugs: Acetoximetilester of Fura-2 (Fura-2/AM), type 1A collagenase, isoproterenol hydrochloride, verapamil hydrochloride, N-2-hydroxyethylpiperazine-N'-2-etanosulfonic acid (HEPES) and trypsin were purchased from Sigma-Aldrich (USA).
0 [micro]M Fura-2 AM (Dojindo, Japan) in HBSS for one hour at room temperature (24 to 26[degrees]C) and washed with HBSS.