We describe a new enzymatic assay for measuring GHb in which Hb in the sample is digested with a specific protease to generate fructosyl amino acid (Fig.
The Hb was resolved by the specific protease, and fructosyl amino acid was separated from Hb in 3-5 min.
The first new technology involves the development of the specific protease, which generates fructosyl amino acid only from Hb in blood.
The second technical advance is the development of FAOD, which reacts only with the fructosyl amino acid generated by the specific protease.
Primary structures of fungal fructosyl amino acid oxidases and their application to the measurement of glycated proteins.
Production of fructosyl lysine oxidase from Fusarium oxysporurn S-1F4 on autoclave-browned medium.
Some plants naturally convert sucrose to fructan by the activity of the enzyme fructosyl transferase.
When they introduced a fructosyl transferase gene into the sugar beet, their theories were confirmed.