restriction length polymorphism

(redirected from fragment length polymorphism)

re·stric·tion length pol·y·mor·phism

, fragment length polymorphism
the existence of allelic forms recognizable by the length of fragments that result when the nucleotide chain is treated by a specific restriction enzyme that cleaves wherever a particular sequence of nucleotides occurs. A mutation in this sequence changes cleaving and hence the number of fragments.
References in periodicals archive ?
Among the PCR based methods PCR-restriction fragment length polymorphism (RFLP) using various genes is one of the options which can be applied for species differentiation.
Amplified fragment length polymorphism and mitochondrial DNA analyses reveal patterns of divergence and hybridization in the hispid cotton rat (Sigmodon hispidus).
ABSTRACT The DNA amplified fragment length polymorphism (AFLP) fingerprinting approach was used to identify a shell color-linked marker in the noble scallop Chlamys nobilis Reeve.
In vitro antifungal susceptibilities and amplified fragment length polymorphism genotyping of a worldwide collection of 350 clinical, veterinary, and environmental Cryptococcus gattii isolates.
PCR and restriction fragment length polymorphism indicated the ompA gene from the isolate was similar to that of avian C.
Variation in restriction fragment length polymorphism could be seen.
Amplification and restriction fragment length polymorphism (RFLP): PCR amplification and RFLP protocols for msp-3[alpha] were reported elsewhere (13).
They cover techniques, laboratory layout, polymerase chain reaction, reaction fragment length polymorphism analysis, single-stranded conformation polymorphism analysis, and sequencing; applications to meat, genetically modified organisms, food allergens, offal, and fish; immunological techniques and antibody-based detection methods; and applications to animal speciation, international food allergen regulations, Japanese regulations and buckwheat allergen detection, and egg, soy, milk, gluten, nut, fish, lupin, mustard, and celery allergen detection.
Using terminal restriction fragment length polymorphism (TRFLP) analysis of PCR-amplified 16S-rDNA sequences, we characterized eubacterial community structure in soil collected on the Bloomington campus of Indiana University beneath native Canadian wildginger (Asarum canadense L.
Morphological analysis included investigating the ratio of three morphometric variables, while the genetic data relied on restriction fragment length polymorphism analysis through the digestion of the mitochondrial sequences of the COI gene.
Essentially, the researchers have sequenced a gene found in all species of Campylobacter and developed polymerase chain reaction (PCR) techniques and PCR-restriction fragment length polymorphism that can be used to detect and differentiate different species of Campylobacter.