Plant extract (6 [micro]l) or 12 [micro]l of a solution of 50 [micro]M fosmidomycin (final concentration in the assay, 1 [micro]M) was added.
03-20 [micro]l of 60 [micro]M fosmidomycin were added instead of plant extract.
However, the compound attracted renewed interest, when it was shown that fosmidomycin acts as a potent inhibitor of IspC protein in the non-mevalonate biosynthetic pathway (Kuzuyama et al.
Fosmidomycin was obtained from Molecular Probes (Invitrogen).
Caesalpiniaceae) (a) The accuracy of the photometric test was tested with the positive reference compound, fosmidomycin (see Fig.
Using next-generation sequencing technology, the research team even found mutations in a gene called PfHAD1, whose dysfunction made malaria resistant to fosmidomycin.
In malaria, the investigational drug, fosmidomycin, blocks isoprenoid synthesis, killing the parasite, but, over time the drug often becomes less effective.
Scientists at the University of Tubingen gave the antibiotic fosmidomycin
to sufferers in Gabon, west Africa.
For example, it has been shown that photosynthesis of leaves of Qu ercus rubra fed an inhibitor of isoprene synthesis, fosmidomycin
, were less able to recover from heat stress induced by exposure 46[degrees]C than were leaves allowed to naturally synthesize isoprene in response to this temperature (Sharkey et al.
The drug, called fosmidomycin, was first synthesized by Fujisawa Pharmaceuticals of Japan.
Whether fosmidomycin or its derivative will kill P.
Wiesner says that the scientists are prepared to test the drugs on malaria patients because the toxicological data on fosmidomycin already indicate that it's safe to use.