In malaria, the investigational drug, fosmidomycin, blocks isoprenoid synthesis, killing the parasite, but, over time the drug often becomes less effective.
Using next-generation sequencing technology, the research team even found mutations in a gene called PfHAD1, whose dysfunction made malaria resistant to fosmidomycin.
Fosmidomycin (FR-31564) had the expected antibacterial spectrum and was selected as a candidate for a DXP reductoisomerase inhibitor.
Fosmidomycin has antibacterial activity against most Gram-negative and some Gram-positive bacteria.
In our assay, fosmidomycin strongly inhibited DXP reductoisomerase in a dose-dependent manner with an [IC.
However, the compound attracted renewed interest, when it was shown that fosmidomycin acts as a potent inhibitor of IspC protein in the non-mevalonate biosynthetic pathway (Kuzuyama et al.
Fosmidomycin was obtained from Molecular Probes (Invitrogen).
03-20 [micro]l of 60 [micro]M fosmidomycin were added instead of plant extract.
Scientists at the University of Tubingen gave the antibiotic fosmidomycin
to sufferers in Gabon, west Africa.
For example, it has been shown that photosynthesis of leaves of Qu ercus rubra fed an inhibitor of isoprene synthesis, fosmidomycin
, were less able to recover from heat stress induced by exposure 46[degrees]C than were leaves allowed to naturally synthesize isoprene in response to this temperature (Sharkey et al.
The drug, called fosmidomycin, was first synthesized by Fujisawa Pharmaceuticals of Japan.
Whether fosmidomycin or its derivative will kill P.