forward-angle light scatter

forward-angle light scatter

The light scattered by a particle in flow cytometry, which is a function of its size. In forward-angle light scatter, the detector is at 180º, or directly in front (forward scatter) of the laser beam.
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The basic measurement device is an optical flow particle analyzer using low forward-angle light scatter as the measurement principle and a 635-nm low-power (5 mW) semiconductor diode laser as the light source [2].
Utilization of the commercially available single-channel Copalis One Immunoassay System showed that HBsAg-mediated binding of colloidal gold causes broadening of the Copalis forward-angle light scatter histogram associated with the HBsAg antibody-coated latex microparticle (Fig.
To detect the leukocyte plot, a dual-parameter analysis (CD53 fluorescence vs forward-angle light scatter and forward- vs large-angle light scatter) was performed on isolated leukocyte samples by using monoclonal mouse anti-human panleukocyte antigen CD53 (Bio-Rad, Segrate, Italy).
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