When the probes are hybridized, the two fluorochromes
A variation of more than 20% in the mean fluorescent index for fluorochrome
-labeled molecules of equivalent soluble fluorochrome
(MESF) calibration beads was observed for instruments that had been standardized using CS&T beads.
, indicating that this heterochromatic block was rich in GC base pairs (Fig.
can emit over a range of wavelengths; thus if one of those wavelengths is the same as that of a second fluorochrome
, spillover occurs.
001% acridine orange, a fluorochrome
that binds nucleic acids, and 0.
This method uses a mixture of two fluorochromes
that stain the nucleic acids and which are excited when exposed to an argon laser at 488 nm which is the cytometer (Molecular Probes, 2001), this permits the analysis of the bacteria on the surface and inside the microcapsules (Demirci et al.
Choosing the right flourochrome: As mentioned above, fluorochromes
differ in the relative brightness of the signal they produce.
To investigate the periodicity of formation of striae and the increment of shell deposited under the different environmental conditions, each scallop was marked individually using the fluorochrome
Briefly, the blastocysts were treated with a permeabilising solution containing the ionic detergent Triton X-100 and the fluorochrome
propidium iodide (cat.
Synthesis of fluorochromes
and pigments in Malassezia furfur by use of tryptophan as the single nitrogen source.
Their compatibility with other popular fluorochromes
and fluorescent protein combinations offers versatility that will further expand researchers' options for multi-color panels.
The factors such as integrity of the blood sample, staining reagents and fluorochromes
, equipment calibration and performance, gating strategies used for the analysis also add to the variation in the CD4+ T cell counts (21-25).