At least 6 individuals per species were subjected to the C-banding and fluorochrome
These mirrors are interference filters that allow certain wavelengths (unique to each fluorochrome
used) to pass through while reflecting others.
Our data from fluorochrome
staining are the first from pholcid spiders.
Six slides from each colony were subjected to sequential fluorochrome
staining Chromomicina A3 (CMA3) and 4'6-diamidino-2-phenylindole (DAPI), which fluoresces when excited by light of a specific wavelength, following Schweizer (1980).
A comparison of fluorochromes
for marking abalone shells.
which first used FITC and Texas Red (6) and then exploded with the use of phycobiliproteins (7)) and in instrumentation (including the introduction of two-laser cytometers capable of simultaneous measurement of three, and then four, independent fluorochromes
were paralleled by clinical research that began to unravel the complexity of normal white blood cell differentiation.
For analysis of Hoechst-33342 and Annexin-V-FITC cell labeling, the Olympus XB51 fluorescence microscope controlled by the Soft Imaging System AnalySIS using the filters appropriate for these fluorochromes
against CK, Her2/neu, aldehyde deyhdrogenase 1 (ALDH1), CD44, and CD24] labeled with fluorochromes
of different colors and spectral image analysis to separate different color spectra.
The degree of separation of the 2 fluorochromes
(in the range of 1 nm) can be estimated from the decrease in donor fluorescence and a corresponding increase in acceptor fluorescence.
A mix of [alpha]-satellite DNA probes (CEE Chromosome Enumeration Probes, Vysis, IL, USA) for chromosomes 18, X, and Y, directly labelled with different fluorochromes
, was used.
The excitation beams of an argon/krypton laser (488 and 568 nm) and band-pass emission filters (522/35 and 605/32 nm) were sequentially used for selective detection of the green (FITC) and red (Texas red) fluorochromes
Cytogenetic analysis of the neotropical spider Nephilengys cruentata (Araneomorphae, Tetragnathidae): Standard staining, Nors, C-Bands and base-specific fluorochromes