In specimens with a normal diploid karyotype, CGH analysis is expected to yield a 1:1 ratio of fluorochromes
for each chromosome.
In an effort to further SMFT technology, he published the formula for the buffered fluorochrome
Quantification can be achieved by addition of a second biotinylated antigen-directed antibody and a third fluorochrome
[phycoerythrin (PE)] coupled to streptavidin.
For multicolor analyses, fluorochrome
compensation was adjusted electronically using normal control peripheral blood leukocytes labeled with FITC-coupled anti-CD4 (Leu-3) and phycoerythrin-coupled anti-CD8 (Leu-2).
Q Can fluorochrome
stains be restained by one of the carbolfuchsin methods?
after C-banding, fluorochrome
and restriction endonuclease treatments.
A third fluorochrome
, phycoerythrin (PE), coupled to streptavidin (SV), quantifies the molecular reaction that has occurred at the microsphere surface.
The use of a fluorochrome
staining procedure with either auramine O or auramine-rhodamine is recommended because it is more sensitive and faster than the Ziehl-Neelson staining technique.
In reference to fluorescence fading method, this technique is based on the fluorescent decay lifetime of the fluorochrome
when this is exposed to excitation light.
Initially, we developed a mathematical model for single-slide hybridization that included both the random variation (attributable to the sequence of a given gene and the amount of a given RNA in our target sample) and the variables introducing artifactual variability: the fluorochrome
, the reverse transcription reaction, and the PCR reaction.
More recent fluorochrome
technologies gave immunologists an excellent method for identifying specific antigens in and on cells.
Preserved samples were stained with fluorochrome
(DAPI or acridine orange) and captured on 0.