fluorescent in situ hybridization


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hybridization

 [hi″brid-ĭ-za´shun]
1. the production of hybrids.
fluorescent in situ hybridization (FISH) a genetic mapping technique using fluorescent tags for analysis of chromosomal aberrations and genetic abnormalities. Called also chromosome painting.
molecular hybridization in molecular biology, formation of a partially or wholly complementary nucleic acid duplex by association of single strands, usually between DNA and RNA strands or previously unassociated DNA strands, but also between RNA strands; used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands.

fluorescent in situ hybridization

a method used to determine the chromosomal location or expression pattern of genomic DNA or cDNA fragments. The piece of DNA to be mapped (the "probe") is labeled with a fluorescent dye and hybridized to a chromosome preparation or to a tissue section. The probe anneals to complementary DNA or RNA sequences. Examination of the chromosomes or tissue section under a fluorescence microscope reveals the number, size, and location of the target sequences.

fluorescent in situ hybridization (FISH)

a genetic mapping technique using fluorescent tags for analysis of chromosomal aberrations and genetic abnormalities. Also called chromosome painting.
Cardiology Finnish Isradipine Study In Hypertension
Molecular medicine Fluorescent In Situ Hybridization. A method for locating a segment of DNA on a chromosome. The DNA is labeled with a fluorescent dye and hybridized to a cytological preparation of chromosomes that has been denatured to allow nucleic acid hybridization between chromosomal DNA and the probe. The site of hybridization is determined by fluorescent microscopy. FISH is a hybrid of 3 technologies: cytogenetics, fluorescence microscopy, and DNA hybridization, which is used to determine cell ploidy and detect chromosome segments by evaluating interphase—non-dividing—nuclei; in FISH, fluoresceinated chromosome probes are used for cytologic analysis and cytogenetic studies, and to detect intratumoral heterogeneity. In genetics, FISH provides a physical mapping approach to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin
DNA probes may be applied to cell preparations on a slide; if the complementary DNA sequence is present, it binds to DNA and can be detected by light microscopy; FISH labels probes nonradioactively either directly with fluorochromes, or indirectly with biotin and fluorochrome-labeled avidin, with digoxeginin and fluorochrome-labeled anti-digoxeginin, or others; the use of multiple band-pass filters allows simultaneous viewing of numerous probes for different chromosomal sequences labeled with different fluorochromes; FISH is useful in cytogenetic studies, where probes for particular chromosomes—e.g., chromosomes 13, 18, 21—or chromosomal regions—e.g., ABL and BCR genes in the Philadelphia translocation—can be used for the prenatal diagnosis of common aneuploidies or to detect early stages of lymphoproliferative disorders; FISH is as sensitive as other analytical techniques—e.g., conventional cytology and flow cytometry, used to diagnose transitional cell carcinoma of the urinary bladder
Pros FISH is simpler, less labor-intensive, and time-consuming—48 hours—than classic cytogenetics—karyotyping—2-3 weeks
Cons Only one question can be asked at a time, i.e., rather than asking ‘global issues’—e.g., what is the genetic composition of a population of cells

fluor·es·cent in si·tu hy·brid·i·za·tion

, fluorescence in situ hybridization (FISH) (flōr-es'ĕnt in sit'ū hī'brid-ī-zā'shŭn, flōr-es'ĕns)
A method used to determine the chromosomal location or expression pattern of genomic DNA or cDNA fragments. The piece of DNA to be mapped (the "probe") is labeled with a fluorescent dye and hybridized to a chromosome preparation or to a tissue section. The probe anneals to complementary DNA or RNA sequences. Examination of the chromosomes or tissue section under a fluorescence microscope reveals the number, size, and location of the target sequences.
References in periodicals archive ?
the "Company") , a leading China-based medical device company that develops, manufactures and markets advanced in-vitro diagnostic products and high intensity focused ultrasound tumor therapy systems, today announced the completion of development of the Prostate Cancer FISH Detection Kit (the "Kit"), a prostate cancer-specific molecular diagnostic test based on the Fluorescent in situ Hybridization ("FISH") technology.
Fluorescent in situ hybridization and enzyme-linked immunosorbent assays are also widely used.
We believe that BioView offers the only automated system that enables laboratories to perform a wide range of genetic tests based on fluorescent in situ hybridization (FISH) technology, combined with morphology or immunohistochemical staining in order to provide important genetic data to the caregiver.
While the labeling in the PlatinumBright kits have been designed to provide the optimal labeling density for Fluorescent in situ hybridization (FISH) applications, the ULS labeling reaction in these kits are also optimal for filter hybridizations such as Northern Blots, Southern Blots, Spot Blots and Dot Blot hybridizations as well as use with Tissue Arrays and ChIP on Chip experiments (labeling of promoter fragments after Chromatin immuno precipitation).
Moore was a central member of the US LABS research and development team and was responsible for the validation and implementation of the US LABS genetics, Fluorescent in situ hybridization and Gene expression microarray programs.
Applications include use in in situ hybridization (ISH), fluorescent in situ hybridization (FISH) and other solid-phase assays where strong signal detection and sensitivity are desired.
Traditional immunodiagnostic products are being replaced by techniques such as in situ hybridization (ISH), fluorescent in situ hybridization (FISH), single-nucleotide polymorphism (SNP) detection, and sequencing technologies that are much more capable of assessing the presence and prognosis of cancer's complex molecular profile.
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