fluorescent antibody technique - definition of fluorescent antibody technique in the Medical dictionary - by the Free Online Medical Dictionary, Thesaurus and Encyclopedia.

technique /tech·nique/ (tek-nēk´) a maneuver, method, or procedure.

dot blot technique a technique for protein detection, analysis, and identification that is similar to the Western blot technique but in which the samples are merely spotted directly onto the membrane through circular templates.

fluorescent antibody technique an immunofluorescence technique in which antigen in tissue sections is located by homologous antibody labeled with fluorochrome or by treating the antigen with unlabeled antibody followed by a second layer of labeled antiglobulin which is reactive with the unlabeled antibody.

isolation-perfusion technique a technique for administering high doses of a chemotherapy agent to a region while protecting the patient from toxicity; the region is isolated and perfused with the drug by means of a pump-oxygenator.

Jerne plaque technique a hemolytic technique for detecting antibody-producing cells: a suspension of presensitized lymphocytes is mixed in an agar gel with erythrocytes; after a period of incubation, complement is added and a clear area of lysis of red cells can be seen around each of the antibody-producing cells.

Mohs' technique microscopically controlled excision of skin cancers in which the tissue to be excised is first fixed in situ with zinc chloride paste (Mohs' chemosurgery), or in which only serial excisions of fresh tissue are used for microscopic analysis (Mohs' surgery) .

Northern blot technique a technique analagous to a Southern blot technique but performed on fragments of RNA.

Pomeroy technique sterilization by ligation of a loop of fallopian tube and resection of the tied loop.

(A), Tube is grasped with forceps; (B), a loop is ligated; (C), the loop is excised; (D), several months later, the fibrosed ends of the tube separate.

Southern blot technique a technique for transferring DNA fragments separated by electrophoresis onto a filter, on which specific fragments can then be detected by their hybridization to defined probes.

Southwestern blot technique a technique analagous to a Southern blot technique but in which proteins are separated by electrophoresis, transferred to a filter, and probed with DNA fragments to identify expression of specific DNA binding proteins.

Western blot technique a technique for analyzing proteins by separating them by electrophoresis, transferring them in place to a filter or membrane, and probing with specific antibodies.

fluorescent antibody technique

Either of two techniques used to test for antigen with a fluorescent antibody: direct, in which immunoglobulin conjugated with a fluorescent dye is added to tissue and combines with a specific antigen; or indirect, in which unlabeled immunoglobulin is added to tissue and combines with a specific antigen, after which the antigen-antibody complex may be labeled with a fluorescent antibody.