1% bovine serum albumin and strained through a 40 gm cell strainer for fluorescence-activated cell sorting
(FACS) (BD Falcon; Becton, Dickinson and Company, Franklin Lakes, NJ, USA).
Effects of knock-down of ZFX on cell cycle progression and apoptosis: To determine the potential mechanism underlying the role of ZFX in the growth of Saos-2 cells the cell cycle profile of si-CTRL and si-ZFX groups were determined by Fluorescence-activated cell sorting
(FACS) analysis 96 hours after infection (Fig.
were the first to identify a highly tumorigenic subpopulation of human breast cancer cells using a set of cell surface markers identified by fluorescence-activated cell sorting
Localization of phytoalexins in fluorescent mesophyll cells isolated from bacterial blight-infected cotton cotyledons and separated from other cells by fluorescence-activated cell sorting
Harnessing the power of fluorescence-activated cell sorting
, the group was able to isolate specific root cells by introducing fluorescent proteins as a means to decipher location and, more importantly, to uncover which genes were expressed during corresponding phases of development.
BD Biosciences manufacturers three systems based on fluorescence-activated cell sorting
(FACS) technology, including the BD FACS VantageTM SE flow cytometry system, BD FACS Calibur[TM] flow cytometry system, and BD[TM] Life Sciences Research (LSR) instrument platforms.
Combining this method with fluorescence-activated cell sorting
provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding.
Using a technique called fluorescence-activated cell sorting
, we can actually sort these oligos we've removed from healthy rats into either progenitors or mature cells.
One of Lederberg's first recruits to the new department was Leonard Herzenberg, PhD, emeritus professor of genetics, who went on to develop the fluorescence-activated cell sorting
machine that opened up new areas of biological research.
Lung progenitor cells (LPCs) were separated by fluorescence-activated cell sorting
Contract award notice: A Fluorescence-activated cell sorting
Fluorescence-activated cell sorting
, a form of flow cytometry, enables researchers to isolate cells on the basis of their protein expression profiles or physical characteristics (such as granularity or size) for subsequent molecular analyses.